LOCUS       62056_456        5302 bp DNA     circular SYN 03-DEC-2019
DEFINITION  Cloning vector Cam_FruR_TAN_CelR_KSL, complete sequence.
ACCESSION   MN207956
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Rondon RE, Groseclose TM, Short AE, Wilson CJ.
  TITLE     Transcriptional programming using engineered systems of 
            transcription factors and genetic architectures
  JOURNAL   Nat Commun 10 (1), 4784 (2019)
  PUBMED    31636266
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Rondon RE, Groseclose T, Short A, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2019) Chemical and Biomolecular Engineering, 
            Georgia Institute of Technology, 950 Atlantic dr. NW, 5110E, 
            Atlanta, GA 30332, United States
REFERENCE   3  (bases 1 to 5302)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2019"; volume: "10"; issue: "1"; pages: "4784"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JUL-2019) Chemical and Biomolecular Engineering, Georgia 
            Institute of Technology, 950 Atlantic dr. NW, 5110E, Atlanta, GA 
            30332, United States"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(27..260)
                     /codon_start=1
                     /transl_table=11
                     /product="rop protein"
                     /label=rop protein
                     /note="maintains plasmids at low copy number"
                     /protein_id="QFU95595.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLQLFN
                     DPALNRRPGRICFRISAIHPLIITYSGVAPGV"
     CDS             complement(371..1384)
                     /codon_start=1
                     /transl_table=11
                     /product="CelR KSL"
                     /label=CelR KSL
                     /protein_id="QFU95596.1"
                     /translation="MKPVTLYDVAEYAGVSKSTVSLVVNQASHVSAKTREKVEAAIKEL
                     GYVPNRAARTLVTRRTDTVALVVSENNQKLFAEPFYAGIVLGVGVALSERGFQFVLATG
                     RSGIEHERLGGYLAGQHVDGVLLLSLHRDDPLPQMLDEAGVPYVYGGRPLGVPEEQVSY
                     VDIDNIGGGRQATQRLIETGHRRIATIAGPQDMVAGVERLQGYREALLAAGMEYDETLV
                     SYGDFTYDSGVAAMRELLDRAPDVDAVFAASDLMGLAALRVLRASGRRVPEDVAVVGYD
                     DSTVAEHAEPPMTSVNQPTELMGREMARLLVDRITGETTEPVRLVLETHLMVRESG"
     promoter        complement(1385..1462)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     regulatory      complement(1477..1554)
                     /label=lacIq promoter
                     /note="lacIq promoter"
                     /regulatory_class="promoter"
     promoter        2163..2240
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             2241..3245
                     /codon_start=1
                     /transl_table=11
                     /product="FruR TAN"
                     /label=FruR TAN
                     /protein_id="QFU95597.1"
                     /translation="MKPVTLYDVAEYAGVSTATVSNVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKSSRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQPD
                     NEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVVGAD
                     QDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYER
                     EAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDF
                     LQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS"
     protein_bind    3258..3279
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      3470..4014
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        4540..4642
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4643..5299
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata
       61 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca
      121 gggtcgttaa atagctgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg
      181 ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg
      241 gttttttcct gtttggtcac tgatgcctcc agcacgtata acgtgctttc ctcgttagaa
      301 tcagagcggg agctaaacag gaggccgatt aaagggattt tagacaggaa cggtacgcca
      361 gaatcctgag tcacccggat tcccgcacca tcaaatgggt ctccagcacc agccgcaccg
      421 gttcggtggt ctccccggtg atccggtcca cgagcagccg ggccatctcc cggcccatca
      481 gctcggtggg ctggttgacg ctggtcatcg gcggttcggc gtgctcggct acggtcgagt
      541 cgtcgtagcc gaccaccgcc acatcctcgg gcacgcggcg tcccgaagca cgcagcaccc
      601 gcagcgcggc cagccccatc aagtcggagg ccgcgaacac ggcgtccacg tcgggggccc
      661 gatccagcag ctcccgcatc gcggccaccc cgctgtcgta ggtgaagtca ccgtagctca
      721 ccagcgtctc gtcgtactcc atccccgcgg cgagcagtgc ttcgcgatac ccctggaggc
      781 gttccacacc agcgaccatg tcctgcgggc ccgcgatcgt agcgatccgc cggtgcccgg
      841 tctcgatcag ccgctgggtg gcctggcgtc ccccgccgat gttgtcgata tcgacatagg
      901 acacctgttc ttcggggacg ccgagcggac ggccgccgta gacgtacggc accccggcct
      961 cgtccagcat ctgcggcagc gggtcgtcgc ggtggagcga cagcaggagg accccgtcga
     1021 cgtgctgtcc ggccaggtag ccgcccagcc gctcatgctc tatcccggag cggcccgtgg
     1081 ccaggacgaa ctggaatccc cgttcggaca gagcaacccc cacgccgagc acgatcccgg
     1141 catagaaggg ttcggcgaag agcttctggt tgttttccga caccaccagg gctacggtgt
     1201 cggtacgtcg ggtgaccagg gtgcgggctg cgcggttcgg cacgtagccc agctctttga
     1261 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacca
     1321 gggaaacggt gcttttagag acaccggcat actctgcgac atcgtataac gttactggtt
     1381 tcatattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
     1441 ttttgcacca ttcgatggtg tcgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1501 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1561 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1621 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1681 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1741 ttaccggata cgtgaccgtg tgcttctcaa atgcctgagg ccagtttgct caggctctcc
     1801 ccgtggaggt aataattgac gatatgatca tttattctgc ctcccagagc ctgataaaaa
     1861 cggttagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc
     1921 gatgcagatc cggaacataa tggtgcaggg cgcttgtttc ggcgtgggta tggtggcagg
     1981 ccccgtggcc gggggactgt tgggcgctgc cggcacctgt cctacgagtt gcatgataaa
     2041 gaagacagtc ataagtgcgg cgacgacacc attccttgcg gcggcggtgc tcaacggcct
     2101 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgagatcc
     2161 cggacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
     2221 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
     2281 gtgtctctac cgcgaccgtt tccaacgtgg tgaaccaggc cagccacgtt tctgcgaaaa
     2341 cgcgggaaaa agtggaagcg gcgatggcgg agctcaatta cattcccaac cgcgtggcac
     2401 aacaactggc gggcaaaagt agccgttcta ttggtcttgt gatccccgat ctggagaaca
     2461 ccagctatac ccgcatcgct aactatcttg aacgccaggc gcggcaacgg ggttatcaac
     2521 tgctgattgc ctgctcagaa gatcagccag acaacgaaat gcggtgcatt gagcaccttt
     2581 tacagcgtca ggttgatgcc attattgttt cgacgtcgtt gcctcctgag catccttttt
     2641 atcaacgctg ggctaacgac ccgttcccga ttgtcgcgct ggaccgcgcc ctcgatcgtg
     2701 aacacttcac cagcgtggtt ggtgccgatc aggatgatgc cgaaatgctg gcggaagagt
     2761 tacgtaagtt tcccgccgag acggtgcttt atcttggtgc gctaccggag ctttctgtca
     2821 gcttcctgcg tgaacaaggt ttccgtactg cctggaaaga tgatccgcgc gaagtgcatt
     2881 tcctgtatgc caacagctat gagcgggagg cggctgccca gttattcgaa aaatggctgg
     2941 aaacgcatcc gatgccgcag gcgctgttca caacgtcgtt tgcgttgttg caaggagtga
     3001 tggatgtcac gctgcgtcgc gacggcaaac tgccttctga cctggcaatt gccacctttg
     3061 gcgataacga actgctcgac ttcttacagt gtccggtgct ggcagtggct caacgtcacc
     3121 gcgatgtcgc agagcgtgtg ctggagattg tcctggcaag cctggacgaa ccgcgtaagc
     3181 caaaacctgg tttaacgcgc attaaacgta atctctatcg ccgcggcgtg ctcagccgta
     3241 gctaagcgca acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg
     3301 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactaac
     3361 atgagaatta caacttatat cgtatggggc tgacttcagg tgctacattt gaagagataa
     3421 attgcactga aatctagaaa tattttatct gattaataag atgatcttct tgagatcgtt
     3481 ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt
     3541 cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga ggagcgcagt
     3601 caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct
     3661 ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac
     3721 tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata
     3781 cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa
     3841 acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac
     3901 gagggagccg ccaggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac
     3961 tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc
     4021 tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc aggaaatctc
     4081 cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag
     4141 tgagcgagga agcggaatat atcctgtatc acatattctg ctgacgcacc ggtgcagcct
     4201 tttttctcct gccacatgaa gcacttcact gacaccctca tcagtgccaa catagtaagc
     4261 cagtatacac tccgctagcg ctgatgtccg gcggtgcttt tgccgttacg caccaccccg
     4321 tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc acctcaaaaa
     4381 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
     4441 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
     4501 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
     4561 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
     4621 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     4681 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     4741 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     4801 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     4861 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     4921 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     4981 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     5041 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     5101 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     5161 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     5221 tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     5281 tgagtggcag ggcggggcgt aa
//
