LOCUS       V017140                 4422 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017140
VERSION     V017140
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             658..1674
                     /gene="RAD51"
                     /label="DNA repair protein RAD51 homolog 1"
                     /note="DNA repair protein RAD51 homolog 1 from Homo
                     sapiens. Accession#: Q06609"
     polyA_signal    1732..1956
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2027..2043)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2051..2067)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2075..2105)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2120..2141)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2429..3017)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3191..4048)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4049..4153)
                     /label="AmpR promoter"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 gcgatgcaga tgcagttgga agcgaatgca gatactagtg tcgaggaaga gtcatttggc
      721 ccgcaaccca tctcgcgttt agagcaatgt ggcatcaatg caaacgatgt gaaaaaatta
      781 gaggaagctg gattccacac ggtcgaagcg gtcgcatacg caccgaaaaa agagctgatc
      841 aacatcaaag gcatcagcga ggcgaaagcc gataagattc ttgcagaggc ggcgaaatta
      901 gttcccatgg gatttacgac ggcgactgag ttccatcaac gtcgttccga gatcattcaa
      961 atcacgaccg gaagcaagga gttggataaa ctgcttcaag gaggcatcga aactggctcg
     1021 atcactgaaa tgtttggaga gtttcgtact ggcaaaaccc agatctgcca cacattggcc
     1081 gtcacttgcc agcttccgat cgatcgtggt gggggcgagg ggaaggccat gtacattgat
     1141 actgagggga cgtttcgtcc cgaacgcctt cttgcggtgg cagagcgtta cggcttgtca
     1201 gggtctgatg ttcttgacaa tgttgcctat gctcgcgcat ttaataccga ccaccagacg
     1261 cagcttctgt accaagcaag cgcgatgatg gtagagtctc gttacgcttt attgattgtt
     1321 gactcagcaa cagcgttgta ccgcacagat tattctgggc gcggcgaatt gtcggcacgc
     1381 caaatgcatt tagcccgctt tttgcgtatg ttgctgcgcc ttgcagacga atttggggtg
     1441 gctgtggtga tcaccaacca agttgtagca caggtggacg gtgccgctat gtttgccgct
     1501 gatccgaaaa aacccatcgg agggaatatt atcgcacatg catcgacaac tcgcttatat
     1561 ctgcgtaagg gccgtggaga aacacgcatc tgcaaaatct atgactcccc atgcctgccg
     1621 gaggcggaag ccatgttcgc aattaacgca gatggcgtag gggacgccaa agattgatga
     1681 ctggtacaca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
     1741 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     1801 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     1861 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     1921 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     1981 ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     2041 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     2101 gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     2161 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2221 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2281 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2341 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2401 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2461 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2521 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2581 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2641 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2701 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2761 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2821 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2881 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2941 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3001 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3061 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3121 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3181 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3241 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3301 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3361 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3421 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3481 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3541 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3601 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3661 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3721 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3781 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3841 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3901 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3961 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4021 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4081 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4141 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     4201 gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc gcatagttaa
     4261 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     4321 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     4381 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg tt
//
