LOCUS       62056_406        3760 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      130..585
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     726..742
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        749..767
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        854..1277
                     /label=AtU6-26
                     /note="Arabidopsis U6-26 gene promoter"
     misc_RNA        1296..1371
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     1428..1444
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1478..1494)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1531..1549)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1570..1586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1594..1610)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1618..1648)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1663..1684)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1972..2561)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2735..3592)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3593..3697)
                     /label=AmpR promoter
ORIGIN
        1 tcttactttt tcatattatt gaagcattta tcaggggtta ttgtctcatg agcggataca
       61 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
      121 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
      181 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
      241 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
      301 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
      361 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
      421 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
      481 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
      541 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttccatt cgccattcag
      601 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
      661 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
      721 acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg aattgggtac
      781 cgagctcgga tccactagta acggccgcca gtgtgctgga attgccctta agcttcgttg
      841 aacaacggaa actcgacttg ccttccgcac aatacatcat ttcttcttag ctttttttct
      901 tcttcttcgt tcatacagtt tttttttgtt tatcagctta cattttcttg aaccgtagct
      961 ttcgttttct tctttttaac tttccattcg gagtttttgt atcttgtttc atagtttgtc
     1021 ccaggattag aatgattagg catcgaacct tcaagaattt gattgaataa aacatcttca
     1081 ttcttaagat atgaagataa tcttcaaaag gcccctggga atctgaaaga agagaagcag
     1141 gcccatttat atgggaaaga acaatagtat ttcttatata ggcccattta agttgaaaac
     1201 aatcttcaaa agtcccacat cgcttagata agaaaacgaa gctgagttta tatacagcta
     1261 gagtcgaagt agtgattggg tcttcgagaa gacctgtttt agagctagaa atagcaagtt
     1321 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttgt
     1381 cccttcgaag ggcaattctg cagatatcca tcacactggc ggccgctcga ggtcgacggt
     1441 atcgataagc ttgatatcga attcctgcag cccgggggat ccactagttc tagagcggcc
     1501 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattt cgagcttggc
     1561 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1621 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     1681 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1741 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1801 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1861 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1921 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1981 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2041 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2101 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2161 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2221 ctgtgtgcac gaaccccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2281 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2341 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2401 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2461 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2521 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2581 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2641 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2701 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2761 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2821 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2881 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2941 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3001 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3061 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3121 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3181 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3241 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3301 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3361 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3421 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3481 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3541 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3601 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3661 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3721 ctgacgcgcc ctgtagcggc gcataagcgc gcgacatcag
//
