LOCUS       40924_265        5310 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector Aqp3a_tVBU1-Strep, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Eskova A, Chauvigne F, Maischein HM, Ammelburg M, Cerda J, 
            Nusslein-Volhard C, Irion U.
  TITLE     Gain-of-function mutations of mau/DrAqp3a influence zebrafish 
            pigment pattern formation through the tissue environment
  JOURNAL   Development (2017) In press
  PUBMED    28506994
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Eskova A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2016) e-CNV group, Max Planck Institute for 
            Developmental Biology, Spemannstr. 35-39, Tuebingen 72076, Germany
REFERENCE   3  (bases 1 to 5310)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5310)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Development
            (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-DEC-2016) e-CNV group, Max Planck Institute for Developmental 
            Biology, Spemannstr. 35-39, Tuebingen 72076, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        422..625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2094..2117
                     /codon_start=1
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
                     /translation="WSHPQFEK"
     CDS             2124..2147
                     /codon_start=1
                     /label=8xHis
                     /note="8xHis affinity tag"
                     /translation="HHHHHHHH"
     polyA_signal    2186..2241
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     promoter        3410..3514
                     /label=AmpR promoter
     CDS             3515..4372
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4546..5134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
       61 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      121 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      181 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      241 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      301 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      361 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      421 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
      481 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      541 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
      601 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcctca ctctcttccg
      661 catcgctgtc tgcgagggcc agctgttggg ctcgcggttg aggacaaact cttcgcggtc
      721 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg
      781 acctgagcga gtccgcatcg accggatcgg aaaacctctc gagaaaggcg tctaaccagt
      841 cacagtcgca aggtaggctg agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt
      901 ttctggcgga ggtgctgctg atgatgtaat taaagtaggc ggtcttgaga cggcggatgg
      961 tcgaggtgag gtgtggcagg cttgagatcc agctgttggg gtgagtactc cctctcaaaa
     1021 gcgggcatta cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc
     1081 acctggcccg atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc
     1141 acaggtgtcc actcccaggt ccaagtttaa acagcggccg catgggttgg cagaaaagcg
     1201 ttctggataa gcttgcgcag actttccaaa tccgcaacaa gttactgcgc cagggattgg
     1261 ctgaatgctt aggaactctc atcctggtga tgtttggctg tggttcattg gcccagttga
     1321 aactaagcga aggttctcat ggtctctttc tcactgcaaa ccttgctttt gggtttggtg
     1381 ctactcttgg aatcttggtt tgcggccagg tgtcaggcgg acatttaaat cctgctgtta
     1441 catttgctct ctgcctcttg ggaagagaaa aatggagaaa gtttcctgtg tactttctgt
     1501 tccaaacact cggatccttc ttgggtgctg ctattatctt tgccgaatac catgacgcaa
     1561 tttatgatta tgctggagaa tcaaatgagt tgcttgtact gggtgaaaaa gaaacagctg
     1621 ggatttttgc tacataccca agcaaatatc tcacccccct aaatggattt tttgaccagg
     1681 tgataggcac agcatccctg attgtgtgca tcctggccat tgtggacccc tacaacaacc
     1741 cgatccctca aggtcttgag gccttcacag tgggattcag cgtccttatc attggtctct
     1801 ccatgggctt caattctggc tatgcagtaa acccagctcg agattttgga cctcttcttt
     1861 ttactgccat ggctggttgg ggtagtgaag tcttcacagc cagggattat tggtttttgg
     1921 tgcccatctt tgctccgttc attggagccg ttattggtgt gattgtgtac cagctgatgg
     1981 tgggatggca tgtggaagga gaggcacgag ataagaaagc taaagctaga gaggaggtga
     2041 tgaacctcaa tgacgtcgcc agcaaggaac tgaattcaag cttgggatcc ggttggagcc
     2101 acccgcagtt cgagaaaacc ggtcatcatc accatcacca tcaccatgga ggacagtgaa
     2161 gatcccccga cctcgacctc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     2221 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttggtcgag
     2281 atccctcgga gatctctagc tagaggatcg atccccgccc cggacgaact aaacctgact
     2341 acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt tggttggtac
     2401 aacttgccaa ctgaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat
     2461 ccagactaac cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt
     2521 agggttagta aaagggtcct aaggaacagc gatgtaggtg ggcgggccaa gataggggcg
     2581 cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg
     2641 ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt
     2701 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     2761 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     2821 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
     2881 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
     2941 tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca
     3001 tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg
     3061 ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     3121 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg
     3181 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     3241 ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gataagctgt caaacatgag
     3301 aattaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     3361 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3421 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3481 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     3541 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     3601 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     3661 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     3721 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     3781 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     3841 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     3901 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     3961 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4021 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     4081 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4141 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4201 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4261 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4321 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4381 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4441 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4501 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4561 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4621 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4681 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4741 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4801 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4861 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4921 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4981 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5041 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5101 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5161 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5221 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     5281 cgagcgcagc gagtcagtga gcgaggaagc
//
