LOCUS       62056_381        5111 bp DNA     circular SYN 03-DEC-2019
DEFINITION  Cloning vector Amp_IA1_YQR_DIMER_RbsR_YQR, complete sequence.
ACCESSION   MN207953
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Rondon RE, Groseclose TM, Short AE, Wilson CJ.
  TITLE     Transcriptional programming using engineered systems of 
            transcription factors and genetic architectures
  JOURNAL   Nat Commun 10 (1), 4784 (2019)
  PUBMED    31636266
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Rondon RE, Groseclose T, Short A, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2019) Chemical and Biomolecular Engineering, 
            Georgia Institute of Technology, 950 Atlantic dr. NW, 5110E, 
            Atlanta, GA 30332, United States
REFERENCE   3  (bases 1 to 5111)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2019"; volume: "10"; issue: "1"; pages: "4784"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JUL-2019) Chemical and Biomolecular Engineering, Georgia 
            Institute of Technology, 950 Atlantic dr. NW, 5110E, Atlanta, GA 
            30332, United States"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             111..299
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    404..546
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        701..805
                     /label=AmpR promoter
     CDS             806..1663
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        1792..1869
                     /label=lacI promoter
     CDS             1870..2868
                     /codon_start=1
                     /transl_table=11
                     /product="RbsR YQR"
                     /label=RbsR YQR
                     /protein_id="QFU95585.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKASHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNS
                     LLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDF
                     EFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELA
                     SFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     protein_bind    2998..3019
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      3227..3815
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3951..4028
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4029..5024
                     /codon_start=1
                     /transl_table=11
                     /product="IA1 YQR Dimer"
                     /label=IA1 YQR Dimer
                     /protein_id="QFU95586.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIASRADQLGASVVVSMVERSGV
                     EACKTAVHTLLAQRVSGLIINYPLDDQEAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTIQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGAVISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLA"
ORIGIN
        1 ctcaggattc tggcgtaccg ttcctgtcta aaatcccttt aatcggcctc ctgtttagct
       61 cccgctctga ttctaacgag gaaagcacgt tatacgtgct ggaggcatca gtgaccaaac
      121 aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg
      181 agaaactcaa cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc
      241 acgctgatga gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
      301 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
      361 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
      421 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
      481 gagagtgcac catatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
      541 atcaggcgct ctctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
      601 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      661 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      721 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      781 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      841 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      901 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
      961 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1021 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1081 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1141 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1201 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1261 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1321 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1381 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1441 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1501 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1561 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1621 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccgtag
     1681 caccctcgca agctcgggca aatcgctgaa tattcgtgct caacggcctc aacctactac
     1741 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc
     1801 gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
     1861 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
     1921 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
     1981 gtggaagcgg cgatggcgga gctcaattac attcccaacc gcgtggcaca acaactggcg
     2041 ggcaaagcga gccataccat tggcatgttg atcactgcca gtaccaatcc tttctattca
     2101 gaactggtgc gtggcgttga acgcagctgc ttcgaacgcg gttatagtct cgtcctttgc
     2161 aataccgaag gcgatgaaca gcggatgaat cgcaatctgg aaacgctgat gcaaaaacgc
     2221 gttgatggct tgctgttact gtgcaccgaa acgcatcaac cttcgcgtga aatcatgcaa
     2281 cgttatccga cagtgcctac tgtgatgatg gactgggctc cgttcgatgg cgacagcgat
     2341 cttattcagg ataactcgtt gctgggcgga gacttagcaa cgcaatatct gatcgataaa
     2401 ggtcataccc gtatcgcctg tattaccggc ccgctggata aaactccggc gcgcctgcgg
     2461 ttggaaggtt atcgggcggc gatgaaacgt gcgggtctca acattcctga tggctatgaa
     2521 gtcactggtg attttgaatt taacggcggg tttgacgcta tgcgccaact gctatcacat
     2581 ccgctgcgtc ctcaggccgt ctttaccgga aatgacgcta tggctgttgg cgtttaccag
     2641 gcgttatatc aggcagagtt acaggttccg caggatatcg cggtgattgg ctatgacgat
     2701 atcgaactgg caagctttat gacgccacca ttaaccacta tccaccaacc gaaagatgaa
     2761 ctgggggagc tggcgattga tgtactcatc catcggataa cccagccgac ccttcagcaa
     2821 caacgattac aacttactcc gattctgatg gaacgcggtt cggcttagct ggtgaaaaga
     2881 aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     2941 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     3001 tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga gagccttcaa
     3061 cccagtcagc tccttccggt gggcgcgggg catgactaac atgagaatta caacttatat
     3121 cgtatggggc tgacttcagg tgctacattt gaagagataa attgctttga ttcgataagc
     3181 agcatcgcct ggcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3241 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3301 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3361 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3421 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3481 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3541 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3601 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3661 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3721 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3781 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3841 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3901 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc gacaccatcg
     3961 aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg
     4021 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc
     4081 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag
     4141 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg
     4201 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc
     4261 aaattgtcgc ggcgattgct tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga
     4321 tggtagaacg aagcggcgtc gaagcctgta aaacggcggt gcacactctt ctcgcgcaac
     4381 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggaagccatt gctgtggaag
     4441 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca
     4501 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg
     4561 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc
     4621 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag
     4681 gcgactggag tgccatgtcc ggttttcaac aaaccataca aatgctgaat gagggcatcg
     4741 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta
     4801 ccgagtccgg gctgcgcgtt ggtgcggtta tctcggtagt gggatacgac gataccgaag
     4861 acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc
     4921 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
     4981 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gtaataaacg caaaccgcct
     5041 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     5101 gcgggcagtg a
//
