LOCUS       62056_371        5090 bp DNA     circular SYN 03-DEC-2019
DEFINITION  Cloning vector Amp_CelR_HQN_GalS_YQR, complete sequence.
ACCESSION   MN207952
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Rondon RE, Groseclose TM, Short AE, Wilson CJ.
  TITLE     Transcriptional programming using engineered systems of 
            transcription factors and genetic architectures
  JOURNAL   Nat Commun 10 (1), 4784 (2019)
  PUBMED    31636266
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Rondon RE, Groseclose T, Short A, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2019) Chemical and Biomolecular Engineering, 
            Georgia Institute of Technology, 950 Atlantic dr. NW, 5110E, 
            Atlanta, GA 30332, United States
REFERENCE   3  (bases 1 to 5090)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2019"; volume: "10"; issue: "1"; pages: "4784"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JUL-2019) Chemical and Biomolecular Engineering, Georgia 
            Institute of Technology, 950 Atlantic dr. NW, 5110E, Atlanta, GA 
            30332, United States"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             111..299
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    404..546
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        701..805
                     /label=AmpR promoter
     CDS             806..1663
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        1792..1869
                     /label=lacI promoter
     CDS             1870..2916
                     /codon_start=1
                     /transl_table=11
                     /product="GalS YQR"
                     /label=GalS YQR
                     /protein_id="QFU95581.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAAQLAGKQSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNL
                     SGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGAGTPD
                     MPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIAR
                     YTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAI
                     TNSTNQAM"
     protein_bind    3046..3067
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      3275..3863
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3999..4076
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4077..5090
                     /codon_start=1
                     /transl_table=11
                     /product="CelR HQN"
                     /label=CelR HQN
                     /protein_id="QFU95582.1"
                     /translation="MKPVTLYDVAEYAGVSHQTVSNVVNQASHVSAKTREKVEAAIKEL
                     GYVPNRAARTLVTRRTDTVALVVSENNQKLFAEPFYAGIVLGVGVALSERGFQFVLATG
                     RSGIEHERLGGYLAGQHVDGVLLLSLHRDDPLPQMLDEAGVPYVYGGRPLGVPEEQVSY
                     VDIDNIGGGRQATQRLIETGHRRIATIAGPQDMVAGVERLQGYREALLAAGMEYDETLV
                     SYGDFTYDSGVAAMRELLDRAPDVDAVFAASDLMGLAALRVLRASGRRVPEDVAVVGYD
                     DSTVAEHAEPPMTSVNQPTELMGREMARLLVDRITGETTEPVRLVLETHLMVRESG"
ORIGIN
        1 ctcaggattc tggcgtaccg ttcctgtcta aaatcccttt aatcggcctc ctgtttagct
       61 cccgctctga ttctaacgag gaaagcacgt tatacgtgct ggaggcatca gtgaccaaac
      121 aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg
      181 agaaactcaa cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc
      241 acgctgatga gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
      301 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
      361 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
      421 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
      481 gagagtgcac catatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
      541 atcaggcgct ctctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
      601 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      661 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      721 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      781 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      841 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      901 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
      961 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1021 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1081 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1141 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1201 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1261 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1321 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1381 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1441 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1501 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1561 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1621 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccgtag
     1681 caccctcgca agctcgggca aatcgctgaa tattcgtgct caacggcctc aacctactac
     1741 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc
     1801 gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
     1861 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
     1921 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
     1981 gtggaagcgg cgatggcgga gctcaattac attcccaacc gcgtggcagc ccaactggcg
     2041 ggcaaacaga gcgacaccat tggcgtggtg gtgatggacg tttctgatgc gtttttcggc
     2101 gcgctggtaa aagcggtgga tctggtcgct cagcagcatc agaaatacgt gctaatcggc
     2161 aatagctatc atgaagcgga aaaagagcgt cacgccattg aggtgttaat tcgccagcgt
     2221 tgtaatgcgt tgattgttca ctcaaaagca ttgagtgacg atgaactggc gcaatttatg
     2281 gataacattc ccggtatggt gttaatcaac cgcgttgtgc cggggtacgc ccatcgttgc
     2341 gtttgcctgg ataatctcag cggtgcccga atggcgacgc gcatgttgct gaataacggt
     2401 catcaacgta ttggttatct ttcttccagc cacggcattg aagatgacgc catgcgtaaa
     2461 gcaggctgga tgagtgcgtt gaaagagcag gatattattc cgccggaaag ctggattggc
     2521 gctggtacgc cggacatgcc gggcggtgag gcggcgatgg ttgaactgct ggggcgcaat
     2581 ctacaactta ccgctgtatt tgcttataac gacaatatgg ccgctggcgc actgacagca
     2641 ttaaaagata atggcattgc gattccgtta catctctcaa tcatcgggtt cgatgatatt
     2701 cccatcgccc gttacaccga cccgcaatta acgaccgtgc gttatcccat tgcttcaatg
     2761 gcgaaattag ccaccgaact ggccttgcag ggggcagcag gcaatattga tcctcgtgcc
     2821 agccactgtt ttatgccgac gttagtgcgt cgccattctg tcgcaacacg ccagaatgcg
     2881 gcggcgatca ctaactcaac aaatcaggcg atgtaactgg tgaaaagaaa aaccaccctg
     2941 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     3001 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct
     3061 cactcattag gcaccgggat ctcgaccgat gcccttgaga gccttcaacc cagtcagctc
     3121 cttccggtgg gcgcggggca tgactaacat gagaattaca acttatatcg tatggggctg
     3181 acttcaggtg ctacatttga agagataaat tgctttgatt cgataagcag catcgcctgg
     3241 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3301 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3361 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3421 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3481 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3541 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3601 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3661 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3721 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3781 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3841 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3901 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3961 gtattaccgc ctttgagtga gctgataccg ctcgccgcga caccatcgaa tggtgcaaaa
     4021 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatatga
     4081 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctctcatcag accgtttcca
     4141 atgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga
     4201 tcaaagagct gggctacgtg ccgaaccgcg cagcccgcac cctggtcacc cgacgtaccg
     4261 acaccgtagc cctggtggtg tcggaaaaca accagaagct cttcgccgaa cccttctatg
     4321 ccgggatcgt gctcggcgtg ggggttgctc tgtccgaacg gggattccag ttcgtcctgg
     4381 ccacgggccg ctccgggata gagcatgagc ggctgggcgg ctacctggcc ggacagcacg
     4441 tcgacggggt cctcctgctg tcgctccacc gcgacgaccc gctgccgcag atgctggacg
     4501 aggccggggt gccgtacgtc tacggcggcc gtccgctcgg cgtccccgaa gaacaggtgt
     4561 cctatgtcga tatcgacaac atcggcgggg gacgccaggc cacccagcgg ctgatcgaga
     4621 ccgggcaccg gcggatcgct acgatcgcgg gcccgcagga catggtcgct ggtgtggaac
     4681 gcctccaggg gtatcgcgaa gcactgctcg ccgcggggat ggagtacgac gagacgctgg
     4741 tgagctacgg tgacttcacc tacgacagcg gggtggccgc gatgcgggag ctgctggatc
     4801 gggcccccga cgtggacgcc gtgttcgcgg cctccgactt gatggggctg gccgcgctgc
     4861 gggtgctgcg tgcttcggga cgccgcgtgc ccgaggatgt ggcggtggtc ggctacgacg
     4921 actcgaccgt agccgagcac gccgaaccgc cgatgaccag cgtcaaccag cccaccgagc
     4981 tgatgggccg ggagatggcc cggctgctcg tggaccggat caccggggag accaccgaac
     5041 cggtgcggct ggtgctggag acccatttga tggtgcggga atccgggtga
//
