LOCUS       62056_361       17251 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17251)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..17251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          153..1169
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     protein_bind    1245..1278
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     protein_bind    1302..1335
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    1502..1636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             2033..3460
                     /codon_start=1
                     /label=tdTomato
                     /note="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    3529..4117
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(4120..4136)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4144..4351
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4359..4392
                     /label=attB
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et
                     al., 2000)"
     promoter        4414..4913
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4973..5773
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5786..6247
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation
                     signal"
     misc_feature    6318..7128
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        10703..11202
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             11229..11801
                     /codon_start=1
                     /label=DTA
                     /note="diphtheria toxin fragment A"
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          11878..11943
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    12017..12241
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(12303..12321)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(12390..12420)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12435..12456)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(12744..13332)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(13506..14363)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(14364..14468)
                     /label=AmpR promoter
     rep_origin      14495..14950
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     15091..15107
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        15117..15135
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    15932..16725
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     enhancer        16821..17124
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgccgggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg
       61 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
      121 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg
      181 tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
      241 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
      301 gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt
      361 tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt
      421 gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
      481 tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct
      541 gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg
      601 gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc
      661 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
      721 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
      781 gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
      841 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
      901 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
      961 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
     1021 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
     1081 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
     1141 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
     1201 ctcatcattt tggcaaagaa ttgatttgat accgcgggcc ctaagaagtt cctattctct
     1261 agaaagtata ggaacttcgt cgacatttaa atcatttaaa tataacttcg tataatgtat
     1321 gctatacgaa gttattcgcg atgaataaat gaaagcttgc agatctgcga ctctagagga
     1381 tctgcgactc tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
     1441 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     1501 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1561 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     1621 ttatcatgtc tggatctgcg actctagagg atcataatca gccataccac atttgtagag
     1681 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     1741 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1801 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1861 ctcatcaatg tatcttatca tgtctggatc cccatcaagc tgatccggaa cccttaatat
     1921 aacttcgtat aatgtatgct atacgaagtt attaggtccc tcgacctgca gcccaagcta
     1981 gatcgaattc ggccggcctt gtacgcgtta agtgcaacac gatcccgcca ccatggtgag
     2041 caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat
     2101 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     2161 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     2221 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta
     2281 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     2341 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
     2401 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
     2461 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
     2521 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat
     2581 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac
     2641 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
     2701 cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca ccgcctcctc
     2761 cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg
     2821 ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     2881 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     2941 cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc
     3001 cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     3061 ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta
     3121 caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac
     3181 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
     3241 gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat
     3301 ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga
     3361 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg
     3421 ccaccacctg ttcctgtacg gcatggacga gctgtacaag taagaattgt gttgcactta
     3481 acgcgtacaa ggccggccct gcaggaattc gatatcaagc ttatcgataa tcaacctctg
     3541 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3601 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3661 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3721 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3781 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3841 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3901 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     3961 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4021 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4081 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgacct
     4141 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     4201 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     4261 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     4321 attgggaaga caatggcagg catgctgggg aactagtggt gccagggcgt gcccttgggc
     4381 tccccgggcg cggcggccgc atcgaattct accgggtagg ggaggcgctt ttcccaaggc
     4441 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     4501 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     4561 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     4621 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     4681 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     4741 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     4801 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     4861 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     4921 atcgccgcta gcgaagttcc tattctctag aaagtatagg aacttcgcca ccatgggatc
     4981 ggccattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     5041 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     5101 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5161 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5221 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5281 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5341 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5401 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5461 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5521 cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5581 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5641 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5701 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5761 tgacgagttc ttctgagggg atccgctgta agtctgcaga aattgatgat ctattaaaca
     5821 ataaagatgt ccactaaaat ggaagttttt cctgtcatac tttgttaaga agggtgagaa
     5881 cagagtacct acattttgaa tggaaggatt ggagctacgg gggtgggggt ggggtgggat
     5941 tagataaatg cctgctcttt actgaaggct ctttactatt gctttatgat aatgtttcat
     6001 agttggatat cataatttaa acaagcaaaa ccaaattaag ggccagctca ttcctcccac
     6061 tcatgatcta tagatctata gatctctcgt gggatcattg tttttctctt gattcccact
     6121 ttgtggttct aagtactgtg gtttccaaat gtgtcagttt catagcctga agaacgagat
     6181 cagcagcctc tgttccacat acacttcatt ctcagtattg ttttgccaag ttctaattcc
     6241 atcagaaagc ttgcagaaga tctccccaac tggggtaacc tttgagttct ctcagttggg
     6301 ggggcgcgcc ggctagaaga tgggcgggag tcttctgggc aggcttaaag gctaacctgg
     6361 tgtgtgggcg ttgtcctgca ggggaattga acaggtgtaa aattggaggg acaagacttc
     6421 ccacagattt tcggttttgt cgggaagttt tttaataggg gcaaataagg aaaatgggag
     6481 gataggtagt catctggggt tttatgcagc aaaactacag gttattattg cttgtgatcc
     6541 gcctcggagt attttccatc gaggtagatt aaagacatgc tcacccgagt tttatactct
     6601 cctgcttgag atccttacta cagtatgaaa ttacagtgtc gcgagttaga ctatgtaagc
     6661 agaattttaa tcatttttaa agagcccagt acttcatatc catttctccc gctccttctg
     6721 cagccttatc aaaaggtatt ttagaacact cattttagcc ccattttcat ttattatact
     6781 ggcttatcca acccctagac agagcattgg cattttccct ttcctgatct tagaagtctg
     6841 atgactcatg aaaccagaca gattagttac atacaccaca aatcgaggct gtagctgggg
     6901 cctcaacact gcagttcttt tataactcct tagtacactt tttgttgatc ctttgccttg
     6961 atccttaatt ttcagtgtct atcacctctc ccgtcaggtg gtgttccaca tttgggccta
     7021 ttctcagtcc agggagtttt acaacaatag atgtattgag aatccaacct aaagcttaac
     7081 tttccactcc catgaatgcc tctctccttt ttctccattt ataaactgag ctattaacca
     7141 ttaatggttt ccaggtggat gtctcctccc ccaatattac ctgatgtatc ttacatattg
     7201 ccaggctgat attttaagac attaaaaggt atatttcatt attgagccac atggtattga
     7261 ttactgctta ctaaaatttt gtcattgtac acatctgtaa aaggtggttc cttttggaat
     7321 gcaaagttca ggtgtttgtt gtctttcctg acctaaggtc ttgtgagctt gtattttttc
     7381 tatttaagca gtgctttctc ttggactggc ttgactcatg gcattctaca cgttattgct
     7441 ggtctaaatg tgattttgcc aagcttcttc aggacctata attttgcttg acttgtagcc
     7501 aaacacaagt aaaatgatta agcaacaaat gtatttgtga agcttggttt ttaggttgtt
     7561 gtgttgtgtg tgcttgtgct ctataataat actatccagg ggctggagag gtggctcgga
     7621 gttcaagagc acagactgct cttccagaag tcctgagttc aattcccagc aaccacatgg
     7681 tggctcacaa ccatctgtaa tgggatctga tgccctcttc tggtgtgtct gaagaccaca
     7741 agtgtattca cattaaataa ataaatcctc cttcttcttc tttttttttt ttttaaagag
     7801 aatactgtct ccagtagaat ttactgaagt aatgaaatac tttgtgtttg ttccaatatg
     7861 gtagccaata atcaaattac tctttaagca ctggaaatgt taccaaggaa ctaattttta
     7921 tttgaagtgt aactgtggac agaggagcca taactgcaga cttgtgggat acagaagacc
     7981 aatgcagact ttaatgtctt ttctcttaca ctaagcaata aagaaataaa aattgaactt
     8041 ctagtatcct atttgtttaa actgctagct ttacttaact tttgtgcttc atctatacaa
     8101 agctgaaagc taagtctgca gccattacta aacatgaaag caagtaatga taattttgga
     8161 tttcaaaaat gtagggccag agtttagcca gccagtggtg gtgcttgcct ttatgccttt
     8221 aatcccagca ctctggaggc agagacaggc agatctctga gtttgagccc agcctggtct
     8281 acacatcaag ttctatctag gatagccagg aatacacaca gaaaccctgt tggggagggg
     8341 ggctctgaga tttcataaaa ttataattga agcattccct aatgagccac tatggatgtg
     8401 gctaaatccg tctacctttc tgatgagatt tgggtattat tttttctgtc tctgctgttg
     8461 gttgggtctt ttgacactgt gggctttctt taaagcctcc ttcctgccat gtggtctctt
     8521 gtttgctact aacttcccat ggcttaaatg gcatggcttt ttgccttcta agggcagctg
     8581 ctgagatttg cagcctgatt tccagggtgg ggttgggaaa tctttcaaac actaaaattg
     8641 tcctttaatt ttttttttaa aaaatgggtt atataataaa cctcataaaa tagttatgag
     8701 gagtgaggtg gactaatatt aaatgagtcc ctcccctata aaagagctat taaggctttt
     8761 tgtcttatac ttaacttttt ttttaaatgt ggtatcttta gaaccaaggg tcttagagtt
     8821 ttagtataca gaaactgttg catcgcttaa tcagattttc tagtttcaaa tccagagaat
     8881 ccaaattctt cacagccaaa gtcaaattaa gaatttctga cttttaatgt taatttgctt
     8941 actgtgaata taaaaatgat agcttttcct gaggcagggt ctcactatgt atctctgcct
     9001 gatctgcaac aagatatgta gactaaagtt ctgcctgctt ttgtctcctg aatactaagg
     9061 ttaaaatgta gtaatacttt tggaacttgc aggtcagatt cttttatagg ggacacacta
     9121 agggagcttg ggtgatagtt ggtaaaatgt gtttcaagtg atgaaaactt gaattattat
     9181 caccgcaacc tactttttaa aaaaaaaagc caggcctgtt agagcatgct taagggatcc
     9241 ctaggacttg ctgagcacac aagagtagtt acttggcagg ctcctggtga gagcatattt
     9301 caaaaaacaa ggcagacaac caagaaacta cagttaaggt tacctgtctt taaaccatct
     9361 gcatatacac agggatatta aaatattcca aataatattt cattcaagtt ttcccccatc
     9421 aaattgggac atggatttct ccggtgaata ggcagagttg gaaactaaac aaatgttggt
     9481 tttgtgattt gtgaaattgt tttcaagtga tagttaaagc ccatgagata cagaacaaag
     9541 ctgctatttc gaggtctctt ggtttatact cagaagcact tctttgggtt tccctgcact
     9601 atcctgatca tgtgctaggc ctaccttagg ctgattgttg ttcaaataaa cttaagtttc
     9661 ctgtcaggtg atgtcatatg atttcatata tcaaggcaaa acatgttata tatgttaaac
     9721 atttgtactt aatgtgaaag ttaggtcttt gtgggtttga tttttaattt tcaaaacctg
     9781 agctaaataa gtcattttta catgtcttac atttggtgga attgtataat tgtggtttgc
     9841 aggcaagact ctctgaccta gtaaccctac ctatagagca ctttgctggg tcacaagtct
     9901 aggagtcaag catttcacct tgaagttgag acgttttgtt agtgtatact agtttatatg
     9961 ttggaggaca tgtttatcca gaagatattc aggactattt ttgactgggc taaggaattg
    10021 attctgatta gcactgttag tgagcattga gtggccttta ggcttgaatt ggagtcactt
    10081 gtatatctca aataatgctg gcctttttta aaaagccctt gttctttatc accctgtttt
    10141 ctacataatt tttgttcaaa gaaatacttg tttggatctc cttttgacaa caatagcatg
    10201 ttttcaagcc atattttttt tccttttttt tttttttttt ggtttttcga gacagggttt
    10261 ctctgtatag ccctggctgt cctggaactc actttgtaga ccaggctggc ctcgaactca
    10321 gaaatccgcc tgcctctgcc tcctgagtgc cgggattaaa ggcgtgcacc accacgcctg
    10381 gctaagttgg atattttgtt atataactat aaccaatact aactccactg ggtggatttt
    10441 taattcagtc agtagtctta agtggtcttt attggccctt cattaaaatc tactgttcac
    10501 tctaacagag gctgttggta ctagtggcac ttaagcaact tcctacggat atactagcag
    10561 attaagggtc agggatagaa actagtctag cgttttgtat acctaccagc tttatactac
    10621 cttgttctga tagaaatatt tcaggacatc tagcttatcg ataccgtcga cggtatcgat
    10681 aagcttgata tcgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
    10741 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
    10801 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
    10861 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
    10921 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
    10981 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
    11041 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
    11101 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
    11161 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag gtcctcgcca
    11221 tggatcctga tgatgttgtt gattcttcta aatcttttgt gatggaaaac ttttcttcgt
    11281 accacgggac taaacctggt tatgtagatt ccattcaaaa aggtatacaa aagccaaaat
    11341 ctggtacaca aggaaattat gacgatgatt ggaaagggtt ttatagtacc gacaataaat
    11401 acgacgctgc gggatactct gtagataatg aaaacccgct ctctggaaaa gctggaggcg
    11461 tggtcaaagt gacgtatcca ggactgacga aggttctcgc actaaaagtg gataatgccg
    11521 aaactattaa gaaagagtta ggtttaagtc tcactgaacc gttgatggag caagtcggaa
    11581 cggaagagtt tatcaaaagg ttcggtgatg gtgcttcgcg tgtagtgctc agccttccct
    11641 tcgctgaggg gagttctagc gttgaatata ttaataactg ggaacaggcg aaagcgttaa
    11701 gcgtagaact tgagattaat tttgaaaccc gtggaaaacg tggccaagat gcgatgtatg
    11761 agtatatggc tcaagcctgt gcaggaaatc gtgtcaggcg atctctttgt gaaggaacct
    11821 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
    11881 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
    11941 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcaga tcctagagct
    12001 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
    12061 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
    12121 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
    12181 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
    12241 gcttctgagg cggaaagaac cagctggggc tcgagggggg gcccggtacc cagcttttgt
    12301 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
    12361 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    12421 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    12481 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    12541 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    12601 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    12661 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    12721 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    12781 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    12841 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    12901 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    12961 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    13021 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    13081 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    13141 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    13201 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    13261 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    13321 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    13381 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    13441 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    13501 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    13561 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    13621 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    13681 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    13741 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    13801 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    13861 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    13921 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    13981 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    14041 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    14101 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    14161 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    14221 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    14281 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    14341 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    14401 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    14461 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
    14521 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
    14581 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
    14641 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    14701 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    14761 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    14821 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
    14881 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
    14941 cttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    15001 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    15061 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
    15121 acgactcact atagggcgaa ttggagctcc accgcggcag gccctccgag cgtggtggag
    15181 ccgttctgtg agacagccgg gtacgagtcg tgacgctgga aggggcaagc gggtggtggg
    15241 caggaatgcg gtccgccctg cagcaaccgg agggggaggg agaagggagc ggaaaagtct
    15301 ccaccggacg cggccatggc tcgggggggg ggggggggca gcggaggagc gcttccggcc
    15361 gacgtctcgt cgctgattgg cttcttttcc tcccgccgtg tgtgaaaaca caaatggcgt
    15421 gttttggttg gcgtaaggcg cctgtcagtt aacggcagcc ggagtgcgca gccgccggca
    15481 gcctcgctct gcccactggg tggggcggga ggtaggtggg gtgaggcgag ctggacgtgc
    15541 gggcgcggtc ggcctctggc ggggcggggg gggggggggg gcagcggagg agcgcttccg
    15601 gccgacgtct cgtcgctgat tggcttcttt tcctcccgcc gtgtgtgaaa acacaaatgg
    15661 cgtgttttgg ttggcgtaag gcgcctgtca gttaacggca gccggagtgc gcagccgccg
    15721 gcagcctcgc tctgcccact gggtggggcg ggaggtaggt ggggtgaggc gagctggacg
    15781 tgcgggcgcg gtcggcctct ggcggggcgg gggggggggg ggggcagcgg aggagcgctt
    15841 ccggccgacg tctcgtcgct gattggcttc ttttcctccc gccgtgtgtg aaaacacaaa
    15901 tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg gcagccggag tgcgcagccg
    15961 ccggcagcct cgctctgccc actgggtggg gcgggaggta ggtggggtga ggcgagctgg
    16021 acgtgcgggc gcggtcggcc tctggcgggg cgggggaggg gagggagggt cagcgaaagt
    16081 agctcgcgcg cgagcggccg cccaccctcc ccttcctctg ggggagtcgt tttacccgcc
    16141 gccggccggg cctcgtcgtc tgattggctc tcggggccca gaaaactggc ccttgccatt
    16201 ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc gggggcggcg aggaggcgct
    16261 cccaggttcc ggccctcccc tcggccccgc gccgcagagt ctggccgcgc gcccctgcgc
    16321 aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag tagggctgag cggctgcggg
    16381 gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag gggcgtgctg agccagacct
    16441 ccatcgcgca ctccggggag tggagggaag gagcgagggc tcagttgggc tgttttggag
    16501 gcaggaagca cttgctctcc caaagtcgct ctgagttgtt atcagtaagg gagctgcagt
    16561 ggagtaggcg gggagaaggc cgcacccttc tccggagggg ggaggggagt gttgcaatac
    16621 ctttctggga gttctctgct gcctcctggc ttctgaggac cgccctgggc ctgggagaat
    16681 cccttccccc tcttccctcg tgatctgcaa ctccagtctt tctagcctta attaaccgtt
    16741 taaacaattc tgcaggaatc tagttattaa tagtaatcaa ttacggggtc attagttcat
    16801 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
    16861 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
    16921 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
    16981 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
    17041 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
    17101 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
    17161 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
    17221 gcgatggggg cggggggggg gggggggcgc g
//
