LOCUS       62056_276        6328 bp DNA     circular SYN 17-JUN-2019
DEFINITION  Cloning vector AbVec6W-hIgLambda, complete sequence.
ACCESSION   MK909076
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Davis CW, Jackson KJL., McElroy AK, Halfmann P, Huang J, Chennareddy
            C, Piper AE, Leung Y, Albarino CG, Crozier I, Ellebedy AH, Sidney J,
            Sette A, Yu T, Nielsen SCA., Goff AJ, Spiropoulou CF, Saphire EO, 
            Cavet G, Kawaoka Y, Mehta AK, Glass PJ, Boyd SD, Ahmed R.
  TITLE     Longitudinal Analysis of the Human B Cell Response to Ebola Virus 
            Infection
  JOURNAL   Cell 177 (6), 1566-1582 (2019)
  PUBMED    31104840
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Davis CW, Ahmed R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2019) Microbiology and Immunology, Emory 
            University, 1462 Clifton Rd, Atlanta, GA 30322, USA
REFERENCE   3  (bases 1 to 6328)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "2019"; volume: "177"; issue: "6"; pages: "1566-1582"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAY-2019) Microbiology and Immunology, Emory University, 1462 
            Clifton Rd, Atlanta, GA 30322, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        818..836
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    961..966
                     /gene="IgL"
                     /label=AgeI site for VL cloning
                     /note="AgeI site for VL cloning"
     V_region        976..1306
                     /gene="IgL"
                     /note="VL from Ebola virus glycoprotein-specific mAb
                     9.6.3A04"
     CDS             1306..1623
                     /label=hIg-lambda-2-CL
                     /note="Human immunoglobulin lambda 2 light chain constant 
                     region"
     misc_feature    1637..2224
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    2331..2379
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(2550..2568)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2573..2589)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2657..3085
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3099..3428
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        3476..3523
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             3542..3937
                     /label=BSD
                     /note="blasticidin S deaminase"
     polyA_signal    4098..4231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4425..5013)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5112..5900)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
      661 gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt gccaagagtg
      721 acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta gaacgcggct
      781 acaattaata cataacctta tgtatcatac acatacgatt taggtgacac tatagaataa
      841 catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc acctcggttc
      901 tatcgattga attccaccat gggatggtca tgtatcatcc tttttctagt agcaactgca
      961 accggttcct gggcccagtc tgccctgact cagcctgcct ccgtgtcggg gtctcctgga
     1021 cagtcgatca ccatctcctg cactggaacc agcagtgatg ttgggagtta taaccttgtc
     1081 tcctggtacc aacagcaccc aggcaaagcc cccaaactca tgatttatga ggtcagtaag
     1141 cggcccccag gggtttctga tcgcttctct ggctccaagt ctggcaacac ggcctccctg
     1201 acaatctctg ggctccaggc tgaggacgag gctgattatt actgctgctc atatgcaagt
     1261 actagcactg tgttattcgg cggagggacc aagctgaccg tcctaggtca gcccaaggct
     1321 gccccctcgg tcactctgtt cccaccctcg agtgaggagc ttcaagccaa caaggccaca
     1381 ctggtgtgtc tcataagtga cttctacccg ggagccgtga cagtggcctg gaaggcagat
     1441 agcagccccg tcaaggcggg agtggagacc accacaccct ccaaacaaag caacaacaag
     1501 tacgcggcca gcagctatct gagcctgacg cctgagcagt ggaagtccca cagaagctac
     1561 agctgccagg tcacgcatga agggagcacc gtggagaaga cagtggcccc tacagaatgt
     1621 tcatagaagc ttgatcctca acctctggat tacaaaattt gtgaaagatt gactggtatt
     1681 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     1741 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     1801 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     1861 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     1921 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     1981 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     2041 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     2101 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     2161 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     2221 ccgcctggat cccgtacaaa gtggttgatc tagagggccc gcggttcgct gatgggggag
     2281 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     2341 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     2401 agggctggca ctctgtcgat accccaccgt gaccccattg gggccaatac gcccgcgttt
     2461 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     2521 tcggggcggc aggccctgcc atagcatccc ctatagtgag tcgtattaca tggtcatagc
     2581 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tggatctgcg cagctggggc
     2641 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     2701 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     2761 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     2821 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     2881 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     2941 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3001 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3061 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     3121 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3181 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3241 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3301 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3361 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     3421 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     3481 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     3541 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     3601 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     3661 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     3721 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     3781 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     3841 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     3901 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcacttcgtg gccgaggagc
     3961 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     4021 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     4081 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4141 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4201 aactcatcaa tgtatcttat catgtctgta taccgtcgct cttccgctgc ttcctcgctc
     4261 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     4321 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4381 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4441 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4501 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4561 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4621 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4681 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4741 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4801 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4861 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4921 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4981 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5041 tctgacgctc agtggaacga cgcgtaactc acgttaaggg attttggtca tgggtggctc
     5101 gacgagggtt atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct
     5161 tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta
     5221 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
     5281 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
     5341 actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
     5401 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
     5461 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
     5521 atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc
     5581 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
     5641 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
     5701 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
     5761 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
     5821 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
     5881 gcgatcaccg cttccctcat aatgtttaac tttgttttag ggcgactgcc ctgctgcgta
     5941 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     6001 gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc
     6061 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     6121 cgctacttgc attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgccttc
     6181 atccgtttcc acggtgtgcg tcacccggca accttgggta gcagcgaagt cgaggcattt
     6241 ctgtcctggc tggtctagaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     6301 cttcgcgatg tacgggccag atatacgc
//
