LOCUS       62056_266        7035 bp DNA     circular SYN 17-JUN-2019
DEFINITION  Cloning vector AbVec6W-hIgG1, complete sequence.
ACCESSION   MK909077
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Davis CW, Jackson KJL., McElroy AK, Halfmann P, Huang J, Chennareddy
            C, Piper AE, Leung Y, Albarino CG, Crozier I, Ellebedy AH, Sidney J,
            Sette A, Yu T, Nielsen SCA., Goff AJ, Spiropoulou CF, Saphire EO, 
            Cavet G, Kawaoka Y, Mehta AK, Glass PJ, Boyd SD, Ahmed R.
  TITLE     Longitudinal Analysis of the Human B Cell Response to Ebola Virus 
            Infection
  JOURNAL   Cell 177 (6), 1566-1582 (2019)
  PUBMED    31104840
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   Davis CW, Ahmed R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2019) Microbiology and Immunology, Emory 
            University, 1462 Clifton Rd, Atlanta, GA 30322, USA
REFERENCE   3  (bases 1 to 7035)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "2019"; volume: "177"; issue: "6"; pages: "1566-1582"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAY-2019) Microbiology and Immunology, Emory University, 1462 
            Clifton Rd, Atlanta, GA 30322, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        818..836
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             919..975
                     /label=IgH signal sequence
                     /note="signal sequence from the mouse immunoglobulin heavy
                     chain variable region"
     V_region        976..1341
                     /gene="IgG1"
                     /note="VH from Ebola virus glycoprotein-specific mAb
                     5.6.1A02"
     CDS             1342..2331
                     /label=hlgG1-CH
                     /note="Human IgG1 heavy chain constant region"
     misc_feature    2344..2931
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    3038..3086
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(3257..3275)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3280..3296)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3364..3792
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3806..4135
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        4183..4230
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             4249..4644
                     /label=BSD
                     /note="blasticidin S deaminase"
     polyA_signal    4805..4938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5132..5720)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5819..6607)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
      661 gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt gccaagagtg
      721 acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta gaacgcggct
      781 acaattaata cataacctta tgtatcatac acatacgatt taggtgacac tatagaataa
      841 catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc acctcggttc
      901 tatcgattga attccaccat gggatggtca tgtatcatcc tttttctagt agcaactgca
      961 accggtgtac attctgaggt gcagctggtg gagtcaggag gaggcttgat ccagcctggg
     1021 gggtccctga gactctcctg tgcagcctct ggtttcgccg tcaggagcaa ctacttgagc
     1081 tgggtccgcc aggctcctgg gaaggggctg gagtgggtct cacttattta tagtggtggt
     1141 ctcacagcct acgcagactc cgtggagggc cggttcacca tctccagaga caattctaag
     1201 aacacactat atcttcaaat gaacagcctg agagtcgagg acacggccct atattactgt
     1261 gcgagagtcg catcatcggc tggaaccttc tactacggta tggacgtctg gggccaaggg
     1321 accacggtca ccgtctcctc agcgtcgacc aagggcccat cggtcttccc cctggcaccc
     1381 tcctccaaga gcacctctgg gggcacagcg gccctgggct gcctggtcaa ggactacttc
     1441 cccgaacctg tgacggtctc gtggaactca ggcgccctga ccagcggcgt gcacaccttc
     1501 ccggctgtcc tacagtcctc aggactctac tccctcagca gcgtggtgac cgtgccctcc
     1561 agcagcttgg gcacccagac ctacatctgc aacgtgaatc acaagcccag caacaccaag
     1621 gtggacaaga aagttgagcc caaatcttgt gacaaaactc acacatgccc accgtgccca
     1681 gcacctgaac tcctgggggg accgtcagtc ttcctcttcc ccccaaaacc caaggacacc
     1741 ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac
     1801 cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc caagacaaag
     1861 ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac
     1921 caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc
     1981 cccatcgaga aaaccatctc caaagccaaa gggcagcccc gagaaccaca ggtgtacacc
     2041 ctgcccccat cccgggatga gctgaccaag aaccaggtca gcctgacctg cctggtcaaa
     2101 ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc ggagaacaac
     2161 tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta cagcaagctc
     2221 accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt gatgcatgag
     2281 gctctgcaca accactacac gcagaagagc ctctccctgt ctccgggtaa atgaagcttg
     2341 atcctcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     2401 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     2461 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     2521 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     2581 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     2641 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     2701 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     2761 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     2821 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     2881 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggatccc
     2941 gtacaaagtg gttgatctag agggcccgcg gttcgctgat gggggaggct aactgaaaca
     3001 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     3061 acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     3121 tgtcgatacc ccaccgtgac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     3181 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     3241 ccctgccata gcatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     3301 ctctggcccg tgtctcaaaa tctctgatgg atctgcgcag ctggggctct agggggtatc
     3361 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3421 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3481 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3541 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3601 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3661 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     3721 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3781 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     3841 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     3901 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3961 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4021 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     4081 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4141 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     4201 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagcct
     4261 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     4321 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     4381 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     4441 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     4501 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     4561 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     4621 ccctctggtt atgtgtggga gggctaagca cttcgtggcc gaggagcagg actgacacgt
     4681 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     4741 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     4801 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4861 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4921 atcttatcat gtctgtatac cgtcgctctt ccgctgcttc ctcgctcact gactcgctgc
     4981 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5041 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5101 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5161 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5221 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5281 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5341 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5401 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5461 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5521 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5581 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5641 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5701 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5761 ggaacgacgc gtaactcacg ttaagggatt ttggtcatgg gtggctcgac gagggttatt
     5821 tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg
     5881 cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga
     5941 cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg
     6001 cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct
     6061 catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa
     6121 atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa
     6181 cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct
     6241 cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag
     6301 ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga
     6361 gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc
     6421 gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct
     6481 tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat
     6541 ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt
     6601 ccctcataat gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc
     6661 tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca
     6721 tagactgtac cccaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac
     6781 caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt
     6841 acagcttacg aaccgaacag gcttatgtcc actgggttcg tgccttcatc cgtttccacg
     6901 gtgtgcgtca cccggcaacc ttgggtagca gcgaagtcga ggcatttctg tcctggctgg
     6961 tctagaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     7021 gggccagata tacgc
//
