LOCUS       62056_261        5080 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        249..628
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        629..832
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        1063..1081
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             1229..1546
                     /codon_start=1
                     /label=mIg-kappa-CL
                     /note="Mouse immunoglobulin kappa light chain constant
                     region"
                     /translation="ADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGS
                     ERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN
                     EC"
     polyA_signal    1628..1762
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1831..2188
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(2208..2224)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2437..2892
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3174..3278
                     /label=AmpR promoter
     CDS             3279..4136
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4310..4898
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat ccaactggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attaattcga
      241 gctcgcccga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      301 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      361 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      421 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      481 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      541 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      601 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      661 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      721 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      781 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      841 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
      901 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa
      961 gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt cgttagaacg
     1021 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
     1081 aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca actgcacctc
     1141 ggttctatcg attgaattcc accatgggat ggtcatgtat catccttttt ctagtagcaa
     1201 ctgcaaccgg ttgactaact agccacgtgc tgatgctgca ccaactgtaa gcatcttccc
     1261 accatccagt gagcagttaa catctggagg tgcctcagtc gtgtgcttct tgaacaactt
     1321 ctaccccaaa gacatcaatg tcaagtggaa gattgatggc agtgaacgac aaaatggcgt
     1381 cctgaacagt tggactgatc aggacagcaa agacagcacc tacagcatga gcagcaccct
     1441 cacgttgacc aaggacgagt atgaacgaca taacagctat acctgtgagg ccactcacaa
     1501 gacatcaact tcacccattg tcaagagctt caacaggaat gagtgttaga gacaaaggtc
     1561 ctgagacgcc accaccagct ccccagctcc atcctatctt cccttctaag cttggccgcc
     1621 atggcccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     1681 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     1741 tgtatcttat catgtctgga tcgggaatta attcggcgca gcaccatggc ctgaaataac
     1801 ctctgaaaga ggaacttggt taggtacctt ctgaggcgga aagaaccagc tgtggaatgt
     1861 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     1921 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     1981 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     2041 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     2101 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     2161 cttttttgga ggcctaggct tttgcaaaaa gctgttaaca gcttggcact ggccgtcgtt
     2221 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     2281 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     2341 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     2401 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     2461 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     2521 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     2581 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     2641 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     2701 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     2761 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
     2821 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     2881 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     2941 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     3001 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     3061 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     3121 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3181 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3241 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     3301 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     3361 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     3421 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     3481 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     3541 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     3601 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3661 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3721 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3781 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     3841 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     3901 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     3961 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4021 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4081 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4141 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4201 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4261 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4321 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4381 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4441 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4501 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4561 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4621 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4681 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4741 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4801 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4861 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4921 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4981 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5041 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
//
