LOCUS       V017128                 8622 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017128
VERSION     V017128
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    232..253
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        268..298
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    306..322
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     330..346
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        367..385
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        413..639
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    867..992
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1485..1718
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1903..1947
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2096..2137
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2214..2331
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2888..2919
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             2981..3697
                     /label="EGFP"
                     /note="enhanced GFP"
     promoter        3794..4151
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4161..4868
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    4878..5466
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5553..5786
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5858..5992
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6019..6154
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6175..6193)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6203..6219)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6361..6816
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6842..6946
                     /label="AmpR promoter"
     CDS             6947..7804
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7978..8566
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      361 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
      421 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
      481 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
      541 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
      601 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
      661 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      721 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      781 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
      841 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
      901 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
      961 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     1021 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     1081 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     1141 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1201 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     1261 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     1321 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1381 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1441 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1501 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1561 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1621 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1681 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1741 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1801 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1861 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1921 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1981 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     2041 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     2101 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     2161 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa
     2221 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2281 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatggatcca
     2341 agctcagatc caagcttgga gcttatgata gcatcttgtt cttagtcttt ttcttaatag
     2401 ggacataaag cccacaaata aaaatatgcc tgaagaatgg gacaggcatt gggcattgtc
     2461 catgcctagt aaagtactcc aagaacctat ttgtatacta gatgacacaa tgttctagcc
     2521 aagcttggag cttatgatag catcttgttc ttagtctttt tcttaatagg gacataaagc
     2581 ccacaaataa aaatatgcct gaagaatggg acaggcattg ggcattgtcc atgcctagta
     2641 aagtactcca agaacctatt tgtatactag atgacacaat gttctagcca agcttatgat
     2701 agcatcttgt tcttagtctt tttcttaata gggacataaa gcccacaaat aaaaatatgc
     2761 ctgaagaatg ggacaggcat tgggcattgt ccatgcctag taaagtactc caagaaccta
     2821 tttgtatact agatgacaca atgttctagg ggtaccgagc tcgagcccgg gctcgagatc
     2881 tagactctag agggtatata atggaagctc gaattccagc ttggcattcc ggtactgttg
     2941 gtaaaaagct tggcattccg gtactgttgg taaagccacc atggtgagca agggcgagga
     3001 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3061 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     3121 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     3181 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     3241 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     3301 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     3361 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     3421 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     3481 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     3541 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     3601 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     3661 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg actctagagt
     3721 cgagaagctt gatgatctgc gcagcaccat ggcctgaaat aacctctgaa agaggaactt
     3781 ggttaggtac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     3841 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3901 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3961 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     4021 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4081 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4141 gcttttgcaa aaagcttacc atggtgagca agggcgagga ggataacatg gccatcatca
     4201 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
     4261 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     4321 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
     4381 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
     4441 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     4501 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     4561 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     4621 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
     4681 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     4741 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
     4801 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     4861 tgtacaagta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4921 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4981 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5041 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5101 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5161 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5221 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     5281 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5341 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5401 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5461 ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
     5521 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5581 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5641 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5701 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5761 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     5821 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     5881 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5941 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6001 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6061 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6121 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     6181 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6241 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6301 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6361 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6421 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6481 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6541 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6601 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6661 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6721 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6781 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6841 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6901 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6961 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7021 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7081 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7141 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7201 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7261 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7321 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7381 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7441 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7501 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7561 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7621 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7681 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7741 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7801 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7861 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7921 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7981 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8041 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8101 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8161 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8221 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8281 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8341 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8401 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8461 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8521 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8581 ggccttttta cggttcctgg ccttttgctg gccttttgct ca
//
