LOCUS       62056_351        2809 bp DNA     circular SYN 12-AUG-2020
DEFINITION  Cloning vector AGG1880:U6-2, complete sequence.
ACCESSION   MT119961
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Tng PYL., Carabajal Paladino L, Verkuijl SAN., Purcell J, Merits A, 
            Leftwich PT, Fragkoudis R, Noad R, Alphey L.
  TITLE     Cas13b-dependent and Cas13b-independent RNA knockdown of viral 
            sequences in mosquito cells following guide RNA expression
  JOURNAL   Commun Biol 3 (1), 413 (2020)
  PUBMED    32737398
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Tng PYL., Carabajal Paladino L, Verkuijl S, Purcell J, Merits A, 
            Leftwich PT, Fragkoudis R, Noad R, Alphey L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2020) Arthropod Genetics Group, The Pirbright 
            Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK
REFERENCE   3  (bases 1 to 2809)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol"; date: "2020"; volume: "3"; issue: "1"; pages: "413"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-FEB-2020) Arthropod Genetics Group, The Pirbright Institute, Ash
            Road, Pirbright, Woking, Surrey GU24 0NF, UK"
FEATURES             Location/Qualifiers
     source          1..2809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      519..1107
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1398..1419
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2192..2296
                     /label=AmpR promoter
     CDS             join(2297..2809,1..345)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
       61 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
      121 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
      181 aaatctggag ccggtgagcg tgggtcgcgc ggtatcattg cagcactggg gccagatggt
      241 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
      301 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
      361 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
      421 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
      481 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
      541 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      601 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      661 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
      721 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
      781 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
      841 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
      901 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      961 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1021 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1081 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1141 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     1201 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     1261 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     1321 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcaattggc
     1381 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccaa acacgggagt
     1441 ctggtaaccc tagtgcacgc aaatatctcg cgggcatatt tggttgctga ggtatattta
     1501 tatttgaacg ccatgagaaa aagcggaaga aattggctca tggccgattt taaggatatt
     1561 taaaaattgt acaatgtaca tataataggc caggagaagt ggatgaactt gtcattcatt
     1621 tttctgtcaa ttctcataca aaatctactt tttctttgac ataaattcac tctaggtgaa
     1681 ccacttcccc tggcctatta aacatccgtt ccttcaatgt gttctttttt ttaagcgtgt
     1741 gttaaaagtt tgctctgctg gtgaattcac gctctacccg ttcaggcagc attcatcgaa
     1801 aagccctatc tgctcgcaca catttacaaa atgctgattg cgttgtgtgc tgaatgggtc
     1861 actcgtccgt cactgcttgc tgtgtacact gtacagttac gcagtctgtg catcgctaga
     1921 atcatattta cggaagagta ttatatatac ccgatgcgtt gctcttcgat tggtactcat
     1981 tcgtagtgcg cattttgcct tcgttgtgga aggtccagtt ttgaggggct attacaactt
     2041 tttttgacac agagcttttg ttaaaaatta tttggcaaaa ataatataat tcggctgcag
     2101 gggcggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     2161 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     2221 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     2281 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     2341 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     2401 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     2461 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     2521 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     2581 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     2641 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     2701 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     2761 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccata
//
