LOCUS       62056_346        2809 bp DNA     circular SYN 12-AUG-2020
DEFINITION  Cloning vector AGG1879:U6-1, complete sequence.
ACCESSION   MT119960
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Tng PYL., Carabajal Paladino L, Verkuijl SAN., Purcell J, Merits A, 
            Leftwich PT, Fragkoudis R, Noad R, Alphey L.
  TITLE     Cas13b-dependent and Cas13b-independent RNA knockdown of viral 
            sequences in mosquito cells following guide RNA expression
  JOURNAL   Commun Biol 3 (1), 413 (2020)
  PUBMED    32737398
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Tng PYL., Carabajal Paladino L, Verkuijl S, Purcell J, Merits A, 
            Leftwich PT, Fragkoudis R, Noad R, Alphey L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2020) Arthropod Genetics Group, The Pirbright 
            Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK
REFERENCE   3  (bases 1 to 2809)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol"; date: "2020"; volume: "3"; issue: "1"; pages: "413"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-FEB-2020) Arthropod Genetics Group, The Pirbright Institute, Ash
            Road, Pirbright, Woking, Surrey GU24 0NF, UK"
FEATURES             Location/Qualifiers
     source          1..2809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      531..1119
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1410..1431
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2204..2308
                     /label=AmpR promoter
     CDS             join(2309..2809,1..357)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
       61 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
      121 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
      181 tttattgctg ataaatctgg agccggtgag cgtgggtcgc gcggtatcat tgcagcactg
      241 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
      301 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
      361 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
      421 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
      481 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      541 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      601 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      661 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
      721 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      781 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      841 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      901 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      961 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1021 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1081 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1141 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1201 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     1261 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     1321 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     1381 aaagcaattg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     1441 aaacacggga gtctggtaac cctagtgcac gcaaatatct cgcgggcata tttggttgct
     1501 gaggtatatt tatatttgaa cgccatgaga aaaagcggaa gaaattggct catggccgat
     1561 tttaaggata tttaaaaatt gtacaatgta catataatag gccaggagaa gtggatgaac
     1621 ttgtcattca tttttctgtc aattctcata caaaatctac tttttctttg acataaattc
     1681 actctaggtg aaccacttcc cctggcctat taaacatccg ttccttcaat gtgttctttt
     1741 ttttaagcgt gtgttaaaag tttgctctgc tggtgaattc acgctctacc cgttcaggca
     1801 gcattcatcg aaaagcccta tctgctcgca cacatttaca aaatgctgat tgcgttgtgt
     1861 gctgaatggg tcactcgtcc gtcactgctt gctgtgtaca ctgtacagtt acgcagtctg
     1921 tgcatcgcta gaatcatatt tacggaagag tattatatat acccgatgcg ttgctcttcg
     1981 attggaagaa gccgcactgc ttcgggtcac cacagttgtg gaaggtccag ttttgagggg
     2041 ctattacaac tttttttgac acagagcttt tgttaaaaat tatttggcaa aaataatata
     2101 attcggctgc aggggcggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2161 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2221 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2281 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     2341 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     2401 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     2461 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     2521 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     2581 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     2641 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     2701 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     2761 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctg
//
