LOCUS       62056_341        2942 bp DNA     circular SYN 12-AUG-2020
DEFINITION  Cloning vector AGG1878:U6-P3, complete sequence.
ACCESSION   MT119952
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Tng PYL., Carabajal Paladino L, Verkuijl SAN., Purcell J, Merits A, 
            Leftwich PT, Fragkoudis R, Noad R, Alphey L.
  TITLE     Cas13b-dependent and Cas13b-independent RNA knockdown of viral 
            sequences in mosquito cells following guide RNA expression
  JOURNAL   Commun Biol 3 (1), 413 (2020)
  PUBMED    32737398
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Tng PYL., Carabajal Paladino L, Verkuijl S, Purcell J, Merits A, 
            Leftwich PT, Fragkoudis R, Noad R, Alphey L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2020) Arthropod Genetics Group, The Pirbright 
            Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK
REFERENCE   3  (bases 1 to 2942)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol"; date: "2020"; volume: "3"; issue: "1"; pages: "413"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-FEB-2020) Arthropod Genetics Group, The Pirbright Institute, Ash
            Road, Pirbright, Woking, Surrey GU24 0NF, UK"
FEATURES             Location/Qualifiers
     source          1..2942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      316..904
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1195..1216
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2122..2226
                     /label=AmpR promoter
     CDS             join(2227..2942,1..142)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtcgcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
       61 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      121 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
      181 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
      241 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
      301 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
      361 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
      421 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
      481 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
      541 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
      601 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      661 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      721 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      781 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      841 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      901 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      961 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1021 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1081 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1141 ctggcacgac aggtttcccg actggaaagc aattggcagt gagcgcaacg caattaatgt
     1201 gagttagctc actcattagg cacccaaaca cgggagtctg gtaaccctag tgcacgcaaa
     1261 tatctcgcgg gcatatttgg ttgctgaggt atatttatat ttgaacgcca tgagaaaaag
     1321 cggaagaaat tggctcatgg ccgattttaa ggatatttaa aaattgtaca atgtacatat
     1381 aataggccag gagaagtgga tgaacttgtc attcattttt ctgtcaattc tcatacaaaa
     1441 tctacttttt ctttgacata aattcactct aggtgaacca cttcccctgg cctattaaac
     1501 atccgttcct tcaatgtgtt ctttttttta agcgtgtgtt aaaagtttgc tctgctggtg
     1561 aattcacgct ctacccgttc aggcagcatt catcgaaaag ccctatctgc tcgcacacat
     1621 ttacaaaatg ctgattgcgt tgtgtgctga atgggtcact cgtccgtcac tgcttgctgt
     1681 gtacactgta cagttacgca gtctgtgcat cgctagaatc atatttacgg aagagtatta
     1741 tatatacccg atgcgttgct cttcgattgg caccacggag gtgatgtggg ccacggcggt
     1801 tgtggaaggt ccagttttga ggggctatta caacggttca cgccgtggaa ggtggacttg
     1861 tgctcgttgt ggaaggtcca gttttgaggg gctattacaa cggtactcat tcgtagtgcg
     1921 cattttgcct tcgttgtgga aggtccagtt ttgaggggct attacaactt tttttgacac
     1981 agagcttttg ttaaaaatta tttggcaaaa ataatataat tcggctgcag gggcggcctc
     2041 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     2101 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2161 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     2221 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     2281 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     2341 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     2401 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     2461 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     2521 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     2581 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     2641 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     2701 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     2761 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     2821 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     2881 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     2941 gg
//
