LOCUS       62056_321        5646 bp DNA     circular SYN 25-APR-2020
DEFINITION  Cloning vector AGG1533, complete sequence.
ACCESSION   MN720705
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Xu X, Yang J, Harvey-Samuel T, Huang Y, Asad M, Chen W, He W, Yang 
            G, Alphey L, You M.
  TITLE     Identification and characterization of the vasa gene in the 
            diamondback moth, Plutella xylostella
  JOURNAL   Insect Biochem. Mol. Biol. (2020) In press
  PUBMED    32283279
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Xu X, Harvey-Samuel T, Alphey L, You M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2019) The Pirbright Insitute, Ash Road, Pirbright,
            Woking GU24 0NF, UK
REFERENCE   3  (bases 1 to 5646)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Insect 
            Biochem. Mol. Biol. (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-NOV-2019) The Pirbright Insitute, Ash Road, Pirbright, Woking 
            GU24 0NF, UK"
FEATURES             Location/Qualifiers
     source          1..5646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      372..912
                     /label=Opie2
                     /note="Opie2"
                     /regulatory_class="promoter"
     intron          931..993
                     /note="Scraps"
     CDS             1021..2802
                     /label=PBase
                     /note="Native piggyBac transposase from Trichoplusia ni"
     polyA_signal    complement(2831..2952)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3454..3470)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3478..3508)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(3523..3544)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3835..4423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4597..5454)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5455..5559)
                     /label=AmpR promoter
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tcatactcgg tggcctcccc accaccaact tttttgcact gcaaaaaaac
      421 acgcttttgc acgcgggccc atacatagta caaactctac gtttcgtaga ctattttaca
      481 taaatagtct acaccgttgt atacgctcca aatacactac cacacattga acctttttgc
      541 agtgcaaaaa agtacgtgtc ggcagtcacg taggccggcc ttatcgggtc gcgtcctgtc
      601 acgtacgaat cacattatcg gaccggacga gtgttgtctt atcgtgacag gacgccagct
      661 tcctgtgttg ctaaccgcag ccggacgcaa ctccttatcg gaacaggacg cgcctccata
      721 tcagccgcgc gttatctcat gcgcgtgacc ggacacgagg cgcccgtccc gcttatcgcg
      781 cctataaata cagcccgcaa cgatctggta aacacagttg aacagcatct gttacagcga
      841 cacaacatga gccggtccaa caacgccaac gcgcccacgc catccaaccg ccgccgcaac
      901 ctgtctctgg tggatccaat tctaattggg gtaagttttc ccgttctttt ctgggttctt
      961 cccttttgct catccttgct gcactacctt caggtgcaag ttgagattca ggaaaccacc
     1021 atgggtagtt ctttagacga tgagcatatc ctctctgctc ttctgcaaag cgatgacgag
     1081 cttgttggtg aggattctga cagtgaaata tcagatcacg taagtgaaga tgacgtccag
     1141 agcgatacag aagaagcgtt tatagatgag gtacatgaag tgcagccaac gtcaagcggt
     1201 agtgaaatat tagacgaaca aaatgttatt gaacaaccag gttcttcatt ggcttctaac
     1261 agaatcttga ccttgccaca gaggactatt agaggtaaga ataaacattg ttggtcaact
     1321 tcaaagtcca cgaggcgtag ccgagtctct gcactgaaca ttgtcagatc tcaaagaggt
     1381 ccgacgcgta tgtgccgcaa tatatatgac ccacttttat gcttcaaact attttttact
     1441 gatgagataa tttcggaaat tgtaaaatgg acaaatgctg agatatcatt gaaacgtcgg
     1501 gaatctatga caggtgctac atttcgtgac acgaatgaag atgaaatcta tgctttcttt
     1561 ggtattctgg taatgacagc agtgagaaaa gataaccaca tgtccacaga tgacctcttt
     1621 gatcgatctt tgtcaatggt gtacgtctct gtaatgagtc gtgatcgttt tgattttttg
     1681 atacgatgtc ttaaaatgga tgacaaaagt atacggccca cacttcgaga aaacgatgta
     1741 tttactcctg ttagaaaaat atgggatctc tttatccatc agtgcataca aaattacact
     1801 ccaggggctc atttgaccat agatgaacag ttacttggtt ttagaggacg gtgtccgttt
     1861 aggatgtata tcccaaacaa gccaagtaag tatggaataa aaatcctcat gatgtgtgac
     1921 agtggtacga agtatatgat aaatggaatg ccttatttgg gaagaggaac acagaccaac
     1981 ggagtaccac tcggtgaata ctacgtgaag gagttatcaa agcctgtgca cggtagttgt
     2041 cgtaatatta cgtgtgacaa ttggttcacc tcaatccctt tggcaaaaaa cttactacaa
     2101 gaaccgtata agttaaccat tgtgggaacc gtgcgatcaa acaaacgcga gataccggaa
     2161 gtactgaaaa acagtcgctc caggccagtg ggaacatcga tgttttgttt tgacggaccc
     2221 cttactctcg tctcatataa accgaagcca gctaagatgg tatacttatt atcatcttgt
     2281 gatgaggatg cttctatcaa cgaaagtacc ggtaaaccgc aaatggttat gtattataat
     2341 caaactaaag gcggagtgga cacgctagac caaatgtgtt ctgtgatgac ctgcagtagg
     2401 aagacgaata ggtggcctat ggcattattg tacggaatga taaacattgc ctgcataaat
     2461 tcttttatta tatacagcca taatgtcagt agcaagggag aaaaggttca aagtcgcaaa
     2521 aaatttatga gaaaccttta catgagcctg acgtcatcgt ttatgcgtaa gcgtttagaa
     2581 gctcctactt tgaagagata tttgcgcgat aatatctcta atattttgcc aaatgaagtg
     2641 cctggtacat cagatgacag tactgaagag ccagtaatga aaaaacgtac ttactgtact
     2701 tactgcccct ctaaaataag gcgaaaggca aatgcatcgt gcaaaaaatg caaaaaagtt
     2761 atttgtcgag agcataatat tgatatgtgc caaagttgtt tctgaggccg gccgcttcga
     2821 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     2881 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     2941 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     3001 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaatctttc tagaagatct
     3061 cctacaatat tctcagctgc catggaaaat cgatgttctt cttttattct ctcaagattt
     3121 tcaggctgta tattaaaact tatattaaga actatgctaa ccacctcatc aggaaccgtt
     3181 gtaggtggcg tgggttttct tggcaatcga ctctcatgaa aactacgagc taaatattca
     3241 atatgttcct cttgaccaac tttattctgc attttttttg aacgaggttt agagcaagct
     3301 tcaggaaact gagacaggaa ttttattaaa aatttaaatt ttgaagaaag ttcagggtta
     3361 atagcatcca ttttttgctt tgcaagttcc tcagcattct taacaaaaga cgtctctttt
     3421 gacatgttta aagtttaaac ctcctgtgtg aaattgttat ccgctcacaa ttccacacat
     3481 tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     3541 attaattgcg ttgcgctcac tgccaattgc tttccagtcg ggaaacctgt cgtgccagct
     3601 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3661 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3721 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3781 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3841 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3901 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3961 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4021 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4081 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4141 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4201 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4261 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4321 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4381 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4441 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4501 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4561 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4621 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4681 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4741 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4801 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4861 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4921 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4981 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5041 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5101 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5161 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     5221 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     5281 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     5341 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     5401 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     5461 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5521 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5581 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     5641 gaggcc
//
