LOCUS       62056_311        2813 bp DNA     circular SYN 12-AUG-2020
DEFINITION  Cloning vector AGG1399, complete sequence.
ACCESSION   MT119950
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Tng PYL., Carabajal Paladino L, Verkuijl SAN., Purcell J, Merits A, 
            Leftwich PT, Fragkoudis R, Noad R, Alphey L.
  TITLE     Cas13b-dependent and Cas13b-independent RNA knockdown of viral 
            sequences in mosquito cells following guide RNA expression
  JOURNAL   Commun Biol 3 (1), 413 (2020)
  PUBMED    32737398
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Tng PYL., Carabajal Paladino L, Verkuijl S, Purcell J, Merits A, 
            Leftwich PT, Fragkoudis R, Noad R, Alphey L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2020) Arthropod Genetics Group, The Pirbright 
            Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK
REFERENCE   3  (bases 1 to 2813)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol"; date: "2020"; volume: "3"; issue: "1"; pages: "413"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-FEB-2020) Arthropod Genetics Group, The Pirbright Institute, Ash
            Road, Pirbright, Woking, Surrey GU24 0NF, UK"
FEATURES             Location/Qualifiers
     source          1..2813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    187..208
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     regulatory      226..760
                     /label=U6-3
                     /note="U6-3"
                     /regulatory_class="promoter"
     misc_feature    762..795
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    796..831
                     /label=PspCas13b guide backbone
                     /note="PspCas13b guide backbone"
     regulatory      832..838
                     /label=U6 terminator
                     /note="U6 terminator"
                     /regulatory_class="terminator"
     promoter        985..1089
                     /label=AmpR promoter
     CDS             1090..1947
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2121..2709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
       61 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      121 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcaattggca gtgagcgcaa
      181 cgcaattaat gtgagttagc tcactcatta ggcacccaaa cacgggagtc tggtaaccct
      241 agtgcacgca aatatctcgc gggcatattt ggttgctgag gtatatttat atttgaacgc
      301 catgagaaaa agcggaagaa attggctcat ggccgatttt aaggatattt aaaaattgta
      361 caatgtacat ataataggcc aggagaagtg gatgaacttg tcattcattt ttctgtcaat
      421 tctcatacaa aatctacttt ttctttgaca taaattcact ctaggtgaac cacttcccct
      481 ggcctattaa acatccgttc cttcaatgtg ttcttttttt taagcgtgtg ttaaaagttt
      541 gctctgctgg tgaattcacg ctctacccgt tcaggcagca ttcatcgaaa agccctatct
      601 gctcgcacac atttacaaaa tgctgattgc gttgtgtgct gaatgggtca ctcgtccgtc
      661 actgcttgct gtgtacactg tacagttacg cagtctgtgc atcgctagaa tcatatttac
      721 ggaagagtat tatatatacc cgatgcgttg ctcttcgatt gagagaccat gtgtctcatc
      781 tatctgctgg tctcagttgt ggaaggtcca gttttgaggg gctattacaa ctttttttga
      841 cacagagctt ttgttaaaaa ttatttggca aaaataatat aattcggctg caggggcggc
      901 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
      961 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     1021 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     1081 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     1141 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1201 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1261 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1321 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1381 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1441 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     1501 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     1561 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     1621 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     1681 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     1741 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     1801 cgtgggtcgc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     1861 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     1921 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     1981 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     2041 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2101 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2161 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2221 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     2281 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2341 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2401 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2461 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2521 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2581 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2641 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2701 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2761 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat tac
//
