LOCUS       62056_286        9195 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1442..1645
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        4532..4743
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4756..5024
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5059..5655
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    5766..5898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7174..7190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7198..7214)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7222..7252)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7267..7288)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7576..8164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8338..9195)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc caaggcctac tagtattatg cccagtacat
     1381 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1441 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1501 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1561 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1621 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     1681 tccacgctgt tttgacctcc atagaagatt ctagagctag cgaattcgcc accatgtccg
     1741 gagggctacc ggtgcctaaa ggcaggcgag aaccccgacc acggctacac cagcttcgat
     1801 tcctttgcct gggcctttct tgcactcttc cgcctgatga cgcaggactg ctgggagcgc
     1861 ctctatcagc agaccctcag gtccgcaggg aagatctaca tgatcttctt catgcttgtc
     1921 atcttcctgg ggtccttcta cctggtgaac ctgatcctgg ccgtggtcgc aatggcctat
     1981 gaggagcaaa accaagccac catcgctgag accgaggaga aggaaaagcg cttccaggag
     2041 gccatggaaa tgctcaagaa agaacacgag gccctcacca tcaggggtgt ggataccgtg
     2101 tcccgtagct ccttggagat gtcccctttg gccccagtaa acagccatga gagaagaagc
     2161 aagaggagaa aacggatgtc ttcaggaact gaggagtgtg gggaggacag gctccccaag
     2221 tctgactcag aagatggtcc cagagcaatg aatcatctca gcctcacccg tggcctcagc
     2281 aggacttcta tgaagccacg ttccagccgc gggagcattt tcacctttcg caggcgagac
     2341 ctgggttctg aagcagattt tgcagatgat gaaaacagca cagcggggga gagcgagagc
     2401 caccacacat cactgctggt gccctggccc ctgcgccgga ccagtgccca gggacagccc
     2461 agtcccggaa cctcggctcc tggccacgcc ctccatggca aaaagaacag cactgtggac
     2521 tgcaatgggg tggtctcatt actgggggca ggcgacccag aggccacatc cccaggaagc
     2581 cacctcctcc gccctgtgat gctagagcac ccgccagaca cgaccacgcc atcggaggag
     2641 ccaggcgggc cccagatgct gacctcccag gctccgtgtg tagatggctt cgaggagcca
     2701 ggagcacggc agcgggccct cagcgcagtc agcgtcctca ccagcgcact ggaagagtta
     2761 gaggagtctc gccacaagtg tccaccatgc tggaaccgtc tcgcccagcg ctacctgatc
     2821 tgggagtgct gcccgctgtg gatgtccatc aagcagggag tgaagttggt ggtcatggac
     2881 ccgtttactg acctcaccat cactatgtgc atcgtactca acacactctt catggcgctg
     2941 gagcactaca acatgacaag tgaattcgag gagatgctgc aggtcggaaa cctggtcttc
     3001 acagggattt tcacagcaga gatgaccttc aagatcattg ccctcgaccc ctactactac
     3061 ttccaacagg gctggaacat cttcgacagc atcatcgtca tccttagcct catggagctg
     3121 ggcctgtccc gcatgagcaa cttgtcggtg ctgcgctcct tccgcctgct gcgggtcttc
     3181 aagctggcca aatcatggcc caccctgaac acactcatca agatcatcgg gaactcagtg
     3241 ggggcactgg ggaacctgac actggtgcta gccatcatcg tgttcatctt tgctgtggtg
     3301 ggcatgcagc tctttggcaa gaactactcg gagctgaggg acagcgactc aggcctgctg
     3361 cctcgctggc acatgatgga cttctttcat gccttcctca tcatcttccg catcctctgt
     3421 ggagagtgga tcgagaccat gtgggactgc atggaggtgt cggggcagtc attatgcctg
     3481 ctggtcttct tgcttgttat ggtcattggc aaccttgtgg tcctgaatct cttcctggcc
     3541 ttgctgctca gctccttcag tgcagacaac ctcacagccc ctgatgagga cagagagatg
     3601 aacaacctcc agctggccct ggcccgcatc cagaggggcc tgcgctttgt caagcggacc
     3661 acctgggatt tctgctgtgg tctcctgcgg cagcggcctc agaagcccgc agcccttgcc
     3721 gcccagggcc agctgcccag ctgcattgcc accccctact ccccgccacc cccagagacg
     3781 gagaaggtgc ctcccacccg caaggaaaca cggtttgagg aaggcgagca accaggccag
     3841 ggcacccccg gggatccaga gcccgtgtgt gtgcccatcg ctgtggccga gtcagacaca
     3901 gatgaccaag aagaagatga ggagaacagc ctgggcacgg aggaggagtc cagcaagcag
     3961 caggaatccc agcctgtgtc cggtggccca gaggcccctc cggattccag gacctggagc
     4021 caggtgtcag cgactgcctc ctctgaggcc gaggccagtg catctcaggc cgactggcgg
     4081 cagcagtgga aagcggaacc ccaggcccca gggtgcggtg agaccccaga ggacagttgc
     4141 tccgagggca gcacagcaga catgaccaac accgctgagc tcctggagca gatccctgac
     4201 ctcggccagg atgtcaagga cccagaggac tgcttcactg aaggctgtgt ccggcgctgt
     4261 ccctgctgtg cggtggacac cacacaggcc ccagggaagg tctggtggcg gttgcgcaag
     4321 acctgctacc acatcgtgga gcacagctgg ttcgagacat tcatcatctt catgatccta
     4381 ctcagcagtg gagcgctggc cttcgaggac atctacctag aggagcggaa gaccatcaag
     4441 gttctgcttg agtatgccga caagatgttc acatatgtct tcgtgctgga gcggccgcga
     4501 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4561 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     4621 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4681 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4741 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     4801 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     4861 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     4921 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     4981 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     5041 cctacgctag acgccaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5101 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5161 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     5221 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     5281 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     5341 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     5401 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     5461 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     5521 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     5581 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     5641 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5701 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     5761 cagttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5821 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5881 tatcttatca tgtctggaat tgactcaaat gatgtcaatt agtctatcag aagctatctg
     5941 gtctcccttc cgggggacaa gacatccctg tttaatattt aaacagcagt gttcccaaac
     6001 tgggttctta tatcccttgc tctggtcaac caggttgcag ggtttcctgt cctcacagga
     6061 acgaagtccc taaagaaaca gtggcagcca ggtttagccc cggaattgac tggattcctt
     6121 ttttagggcc cattggtatg gctttttccc cgtatccccc caggtgtctg caggctcaaa
     6181 gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg
     6241 tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg
     6301 gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg
     6361 ggggggctgt ccctgatatc tataacaaga aaatatatat ataataagtt atcacgtaag
     6421 tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag
     6481 ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc
     6541 gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca
     6601 gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt
     6661 ttacgcagac tatctttcta gggttaatct agctgcatca ggatcatatc gtcgggtctt
     6721 ttttccggct cagtcatcgc ccaagctggc gctatctggg catcggggag gaagaagccc
     6781 gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat gactgctgct
     6841 gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt gtggccatgc
     6901 acgcctttaa cggtgaactg ttcgttcagg ccacctggga taccagttcg tcgcggcttt
     6961 tccggacaca gttccggatg gtcagcccga agcgcatcag caacccgaac aataccggcg
     7021 acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt
     7081 acgtcagcga ggacgggtat cctggctgga tgccgcagaa atggacatgg ataccccgtg
     7141 agttacccgg cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7201 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7261 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7321 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7381 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7441 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7501 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7561 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7621 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7681 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7741 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7801 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7861 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7921 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7981 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     8041 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8101 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8161 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8221 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8281 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8341 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8401 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8461 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8521 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8581 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8641 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8701 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8761 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8821 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8881 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8941 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9001 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9061 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9121 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9181 atgttgaata ctcat
//
