LOCUS       V010517                12382 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V010517
VERSION     V010517
KEYWORDS    AAVS1-Blasticidin-CAG-Flpe-ERT2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12382)
  AUTHORS   Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z,
            Zhang SC
  TITLE     Engineering Human Stem Cell Lines with Inducible Gene Knockout using
            CRISPR/Cas9.
  JOURNAL   Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi:
            10.1016/j.stem.2015.06.001.
   PUBMED   26145478
REFERENCE   2  (bases 1 to 12382)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 12382)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem
            Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi:
            10.1016/j.stem.2015.06.001."
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..836
                     /label="HA-L"
                     /note="left homology arm from the adeno-associated virus
                     integration site (AAVS1) within intron 1 of the human
                     PPP1R12C gene"
     misc_feature    843..868
                     /label="SA"
                     /note="splice acceptor site"
     CDS             892..945
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             955..1374
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     polyA_signal    1424..1648
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        1716..2095
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2097..2374
                     /label="chicken beta-actin promoter"
     intron          2376..3393
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     primer_bind     3401..3420
                     /label="pCAG-F"
                     /note="Rabbit beta-globin intron, for pCAG plasmids,
                     forward primer"
     regulatory      3457..3466
                     /label="Kozak sequence"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3469..4737
                     /label="FLPo"
                     /note="nuclear-targeted site-specific recombinase"
     CDS             4762..5697
                     /label="ERT2"
                     /note="mutated ligand-binding domain of the human estrogen
                     receptor (Feil et al., 1997)"
     misc_feature    5737..6325
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    6357..6833
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6943..6962)
                     /label="Bglob-pA-R"
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7008..7063
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(7062..7081)
                     /label="rbglobpA-R"
                     /note="Rabbit beta-globin polyA, reverse primer. Also
                     called rb-glob-pA-term-R"
     primer_bind     complement(7424..7440)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    7448..7464
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7472..7502)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7517..7538)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    7612..8448
                     /label="HA-R"
                     /note="right homology arm from the adeno-associated virus
                     integration site (AAVS1) within intron 1 of the human
                     PPP1R12C gene"
     promoter        complement(8489..8507)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8514..8531)
                     /label="M13 Forward"
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21)
                     Forward"
     primer_bind     complement(8514..8530)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(8523..8545)
                     /label="M13/pUC Forward"
                     /note="In lacZ gene"
     CDS             8668..8967
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     primer_bind     complement(9373..9392)
                     /label="Neo-R"
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9987..10006
                     /label="Neo-F"
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(10370..11227)
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      11351..11939
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     12093..12110
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     protein_bind    12227..12248
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        12263..12293
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    12301..12317
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12325..12341
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        12362..12380
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc
       61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca
      121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc
      181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc
      241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc
      301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg
      361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc
      421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc
      481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc
      541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta
      601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag
      661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt
      721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt
      781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc
      841 ttctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga
      901 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggac cggtatgaaa
      961 acatttaaca tttctcaaca agatctagaa ttagtagaag tagcgacaga gaagattaca
     1021 atgctttatg aggataataa acatcatgtg ggagcggcaa ttcgtacgaa aacaggagaa
     1081 atcatttcgg cagtacatat tgaagcgtat ataggacgag taactgtttg tgcagaagcc
     1141 attgcgattg gtagtgcagt ttcgaatgga caaaaggatt ttgacacgat tgtagctgtt
     1201 agacaccctt attctgacga agtagataga agtattcgag tggtaagtcc ttgtggtatg
     1261 tgtagggagt tgatttcaga ctatgcacca gattgttttg tgttaataga aatgaatggc
     1321 aagttagtca aaactacgat tgaagaactc attccactca aatatacccg aaattaatga
     1381 gaattcgggg atcaattctc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
     1441 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     1501 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     1561 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     1621 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
     1681 actagagctt gcggaaccct taatggtacc gtctcgacat tgattattga ctagttatta
     1741 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     1801 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     1861 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     1921 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     1981 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     2041 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga
     2101 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt
     2161 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcg
     2221 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
     2281 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
     2341 gcggccctat aaaaagcgaa gcgcgcggcg ggcggggagt cgctgcgacg ctgccttcgc
     2401 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
     2461 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
     2521 taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc
     2581 cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
     2641 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
     2701 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
     2761 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     2821 gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac
     2881 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc
     2941 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
     3001 tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     3061 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     3121 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag
     3181 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     3241 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg
     3301 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
     3361 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
     3421 ctgtctcatc attttggcaa agaattcgtc gacgccgcca ccatggctat gagccagttc
     3481 gacatcctgt gcaagacccc ccccaaggtg ctggtgcggc agttcgtgga gagattcgag
     3541 aggcccagcg gcgagaagat cgccagctgt gccgccgagc tgacctacct gtgctggatg
     3601 atcacccaca acggcaccgc catcaagagg gccaccttca tgagctacaa caccatcatc
     3661 agcaacagcc tgagcttcga catcgtgaac aagagcctgc agttcaagta caagacccag
     3721 aaggccacca tcctggaggc cagcctgaag aagctgatcc ccgcctggga gttcaccatc
     3781 atcccttaca acggccagaa gcaccagagc gacatcaccg acatcgtgtc cagcctgcag
     3841 ctgcagttcg agagcagcga ggaggccgac aagggcaaca gccacagcaa gaagatgctg
     3901 aaggccctgc tgtccgaggg cgagagcatc tgggagatca ccgagaagat cctgaacagc
     3961 ttcgagtaca ccagcaggtt caccaagacc aagaccctgt accagttcct gttcctggcc
     4021 acattcatca actgcggcag gttcagcgac atcaagaacg tggaccccaa gagcttcaag
     4081 ctggtgcaga acaagtacct gggcgtgatc attcagtgcc tggtgaccga gaccaagaca
     4141 agcgtgtcca ggcacatcta ctttttcagc gccagaggca ggatcgaccc cctggtgtac
     4201 ctggacgagt tcctgaggaa cagcgagccc gtgctgaaga gagtgaacag gaccggcaac
     4261 agcagcagca acaagcagga gtaccagctg ctgaaggaca acctggtgcg cagctacaac
     4321 aaggccctga agaagaacgc cccctacccc atcttcgcta tcaagaacgg ccctaagagc
     4381 cacatcggca ggcacctgat gaccagcttt ctgagcatga agggcctgac cgagctgaca
     4441 aacgtggtgg gcaactggag cgacaagagg gcctccgccg tggccaggac cacctacacc
     4501 caccagatca ccgccatccc cgaccactac ttcgccctgg tgtccaggta ctacgcctac
     4561 gaccccatca gcaaggagat gatcgccctg aaggacgaga ccaaccccat cgaggagtgg
     4621 cagcacatcg agcagctgaa gggcagcgcc gagggcagca tcagataccc cgcctggaac
     4681 ggcatcatca gccaggaggt gctggactac ctgagcagct acatcaacag gcggatcacc
     4741 ggttgcgtac gcggatcctc tgctggagac atgagagctg ccaacctttg gccaagcccg
     4801 ctcatgatca aacgctctaa gaagaacagc ctggccttgt ccctgacggc cgaccagatg
     4861 gtcagtgcct tgttggatgc tgagcccccc atactctatt ccgagtatga tcctaccaga
     4921 cccttcagtg aagcttcgat gatgggctta ctgaccaacc tggcagacag ggagctggtt
     4981 cacatgatca actgggcgaa gagggtgcca ggctttgtgg atttgaccct ccatgatcag
     5041 gtccaccttc tagaatgtgc ctggctagag atcctgatga ttggtctcgt ctggcgctcc
     5101 atggagcacc cagtgaagct actgtttgct cctaacttgc tcttggacag gaaccaggga
     5161 aaatgtgtag agggcatggt ggagatcttc gacatgctgc tggctacatc atctcggttc
     5221 cgcatgatga atctgcaggg agaggagttt gtgtgcctca aatctattat tttgcttaat
     5281 tctggagtgt acacatttct gtccagcacc ctgaagtctc tggaagagaa ggaccatatc
     5341 caccgagtcc tggacaagat cacagacact ttgatccacc tgatggccaa ggcaggcctg
     5401 accctgcagc agcagcacca gcggctggcc cagctcctcc tcatcctctc ccacatcagg
     5461 cacatgagta acaaaggcat ggagcatctg tacagcatga agtgcaagaa cgtggtgccc
     5521 ctctatgacc tgctgctgga ggcggcggac gcccaccgcc tacatgcgcc cactagccgt
     5581 ggaggggcat ccgtggagga gacggaccaa agccacttgg ccactgcggg ctctacttca
     5641 tcgcattcct tgcaaaagta ttacatcacg ggggaggcag agggtttccc tgccacagct
     5701 tgatagacgc gtgaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     5761 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5821 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5881 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5941 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6001 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6061 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6121 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct atgttgccac ctggattctg
     6181 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6241 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6301 atctcccttt gggccgcctc cccgcatcga taccgagcgc tgctcgagag atctacgggt
     6361 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc
     6421 ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct
     6481 ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct
     6541 gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca
     6601 ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg
     6661 gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt
     6721 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc
     6781 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt
     6841 tgtaggtaac caggcgcgtg aattcactcc tcaggtgcag gctgcctatc agaaggtggt
     6901 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
     6961 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     7021 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     7081 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     7141 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     7201 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     7261 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     7321 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     7381 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg
     7441 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7501 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7561 ctttccagtc gggaaacctg tcgtgccagc ggatcgacag tactaagctt tactagggac
     7621 aggattggtg acagaaaagc cccatcctta ggcctcctcc ttcctagtct cctgatattg
     7681 ggtctaaccc ccacctcctg ttaggcagat tccttatctg gtgacacacc cccatttcct
     7741 ggagccatct ctctccttgc cagaacctct aaggtttgct tacgatggag ccagagagga
     7801 tcctgggagg gagagcttgg cagggggtgg gagggaaggg ggggatgcgt gacctgcccg
     7861 gttctcagtg gccaccctgc gctaccctct cccagaacct gagctgctct gacgcggctg
     7921 tctggtgcgt ttcactgatc ctggtgctgc agcttcctta cacttcccaa gaggagaagc
     7981 agtttggaaa aacaaaatca gaataagttg gtcctgagtt ctaactttgg ctcttcacct
     8041 ttctagtccc caatttatat tgttcctccg tgcgtcagtt ttacctgtga gataaggcca
     8101 gtagccagcc ccgtcctggc agggctgtgg tgaggagggg ggtgtccgtg tggaaaactc
     8161 cctttgtgag aatggtgcgt cctaggtgtt caccaggtcg tggccgcctc tactcccttt
     8221 ctctttctcc atccttcttt ccttaaagag tccccagtgc tatctgggac atattcctcc
     8281 gcccagagca gggtcccgct tccctaaggc cctgctctgg gcttctgggt ttgagtcctt
     8341 ggcaagccca ggagaggcgc tcaggcttcc ctgtccccct tcctcgtcca ccatctcatg
     8401 cccctggctc tcctgcccct tccctacagg ggttcctggc tctgctctaa gggcaagggc
     8461 gaattcgcgg ccgctaaatt caattcgccc tatagtgagt cgtattacaa ttcactggcc
     8521 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     8581 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     8641 caacagttgc gcagcctata cgtacggcag tttaaggttt acacctataa aagagagagc
     8701 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgccggg gcgacggatg
     8761 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     8821 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     8881 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     8941 gccattaacc tgatgttctg gggaatataa atgtcaggca tgagattatc aaaaaggatc
     9001 ttcacctaga tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg
     9061 aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta
     9121 gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa
     9181 ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
     9241 atggctttct tgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca
     9301 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     9361 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     9421 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     9481 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
     9541 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     9601 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     9661 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     9721 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     9781 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     9841 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atgcatggcg atgcctgctt
     9901 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     9961 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
    10021 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
    10081 catcgccttc tatcgccttc ttgacgagtt cttctgaatt attaacgctt acaatttcct
    10141 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatca ggtggcactt
    10201 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    10261 atccgctcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10321 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10381 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10441 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10501 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10561 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10621 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10681 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10741 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10801 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10861 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10921 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10981 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    11041 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    11101 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    11161 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    11221 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    11281 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    11341 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    11401 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    11461 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    11521 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    11581 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    11641 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    11701 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    11761 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    11821 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    11881 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    11941 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    12001 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    12061 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    12121 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    12181 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    12241 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    12301 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
    12361 gaattaaccc tcactaaagg gt
//
