LOCUS       Exported                3798 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector AAV-hSyn-Jaws-mRuby2-8X2C, complete sequence.
ACCESSION   MH458081
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Tseng H-a., Ta T, Gritton HJ, Man H-Y.
  TITLE     A microRNA-based gene targeting tool for virally labeling 
            interneurons in the rodent cortex
  JOURNAL   Cell Rep (2018) In press
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Keaveney MK, Han X.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2018) Biomedical Engineering, Boston University, 
            610 Commonwealth Ave, Boston, MA 02215, USA
REFERENCE   3  (bases 1 to 3798)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: APE Plasmid Editor v. v2.0.53.c
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Cloning vector AAV-hSyn-Jaws-mRuby2-8X2C"
                     /mol_type="other DNA"
                     /db_xref="taxon:2250249"
     repeat_region   1..101
                     /label=5'ITR
                     /note="5'ITR"
     regulatory      197..677
                     /regulatory_class="promoter"
                     /note="hSyn"
     promoter        224..671
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      684..692
                     /regulatory_class="ribosome_binding_site"
     CDS             696..2270
                     /codon_start=1
                     /product="Jaws-mRUby2-ER2"
                     /label=Jaws-mRUby2-ER2
                     /protein_id="AWX65709.1"
                     /translation="TAVSTTATTVLQATQSDVLQEIQSNFLLNSSIWVNIALAGVVILL
                     FVAMGRDLESPRAKLIWVATMLVPLVSISSYAGLASGLTVGFLQMPPGHALAGQEVLSP
                     WGRYLTWTFSTPMILLALGLLADTDIASLFTAITMDIGMCVTGLAAALITSSHLLRWVF
                     YGISCAFFVAVLYVLLVQWPADAEAAGTSEIFGTLRILTVVLWLGYPILFALGSEGVAL
                     LSVGVTSWGYSGLDILAKYVFAFLLLRWVAANEGTVSGSGMGIGSGGAAPADDRPVVSK
                     GEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMRIKVIEGGPLPFAFDILA
                     TSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVVTVMQDTSLEDGCLVYH
                     VQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHMALKVDGGGHLSCSFVTT
                     YRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFAGLGGGMDELYKLYK
                     FCYENEV"
     CDS             1530..2237
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     ncRNA           complement(2270..2285)
                     /label=miRNA
                     /note="microRNA"
                     /ncRNA_class="miRNA"
     misc_feature    2748..3336
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    2750..3337
                     /label=WPRE
                     /note="WPRE"
     CDS             complement(3219..3230)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3339..3355)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3385..3506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3609..3798
                     /label=3'ITR
                     /note="3'ITR"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatct gaattctcta gactgcagag ggccctgcgt atgagtgcaa gtgggtttta
      241 ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac ccactggaca
      301 agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg aggggaaaca
      361 ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg ccttcgcccc
      421 cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg tcactcgccg
      481 gtcccccgca aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag
      541 ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg
      601 gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc
      661 tgagagcgca gtcgagagga tccgccgcca ccatgaccgc cgtgagcacc acagccacta
      721 ccgtgctgca ggccacacag agcgacgtgc tgcaggagat ccagtccaac ttcctgctga
      781 atagctccat ctgggtgaac attgctctgg ccggagtggt catcctgctg tttgtggcca
      841 tggggaggga tctggaatcc cctagagcta agctgatctg ggtggccaca atgctggtgc
      901 cactggtgtc tatttctagt tacgctggac tggccagtgg gctgactgtg ggcttcctgc
      961 agatgccacc tggacacgct ctggccggac aggaggtgct gagcccatgg ggccggtatc
     1021 tgacatggac tttctccact cccatgatcc tgctggctct gggactgctg gccgacaccg
     1081 atattgccag cctgttcacc gccatcacaa tggacattgg catgtgcgtg acaggactgg
     1141 ccgctgccct gatcactagc tcccatctgc tgcgctgggt gttctacgga atttcttgtg
     1201 ctttctttgt ggccgtgctg tatgtgctgc tggtgcagtg gccagctgat gctgaggctg
     1261 ctgggaccag tgaaatcttt ggcactctga ggattctgac cgtggtgctg tggctggggt
     1321 accctatcct gttcgctctg ggctctgagg gagtggccct gctgagtgtg ggagtgacca
     1381 gctggggata ctccggactg gacatcctgg ctaaatacgt gttcgccttt ctgctgctga
     1441 gatgggtggc tgccaatgaa ggcacagtgt ctgggagtgg aatgggaatc gggtccggag
     1501 gagctgctcc agccgacgat cgaccggtag tgtctaaggg cgaagagctg atcaaggaaa
     1561 atatgcgtat gaaggtggtc atggaaggtt cggtcaacgg ccaccaattc aaatgcacag
     1621 gtgaaggaga aggcaatccg tacatgggaa ctcaaaccat gaggatcaaa gtcatcgagg
     1681 gaggacccct gccatttgcc tttgacattc ttgccacgtc gttcatgtat ggcagccgta
     1741 cttttatcaa gtacccgaaa ggcattcctg atttctttaa acagtccttt cctgagggtt
     1801 ttacttggga aagagttacg agatacgaag atggtggagt cgtcaccgtc atgcaggaca
     1861 ccagccttga ggatggctgt ctcgtttacc acgtccaagt cagaggggta aactttccct
     1921 ccaatggtcc cgtgatgcag aagaagacca agggttggga gcctaataca gagatgatgt
     1981 atccagcaga tggtggtctg aggggataca ctcatatggc actgaaagtt gatggtggtg
     2041 gccatctgtc ttgctctttc gtaacaactt acaggtcaaa aaagaccgtc gggaacatca
     2101 agatgcccgg tatccatgcc gttgatcacc gcctggaaag gttagaggaa agtgacaatg
     2161 aaatgttcgt agtacaacgc gaacacgcag ttgccaagtt cgccgggctt ggtggtggga
     2221 tggacgagct gtacaagctg tacaagttct gctacgagaa tgaagtgtaa actagtgcgg
     2281 ccttaaagag accggttcac tgtgacagta aaagagaccg gttcactgtg agaatgaaag
     2341 agaccggttc actgtgatcg gaaaagagac cggttcactg tgagcggcct taaagagacc
     2401 ggttcactgt gacagtaaaa gagaccggtt cactgtgaga atgaaagaga ccggttcact
     2461 gtgatcggaa aagagaccgg ttcactgtga gcggccttga aacccagcag acaatgtagc
     2521 tcagtagaaa cccagcagac aatgtagctg aatggaaacc cagcagacaa tgtagcttcg
     2581 gagaaaccca gcagacaatg tagctgcggc cttgaaaccc agcagacaat gtagctcagt
     2641 agaaacccag cagacaatgt agctgaatgg aaacccagca gacaatgtag cttcggagaa
     2701 acccagcaga caatgtagct gcggccgcga ctctaaagct tatcgataat caacctctgg
     2761 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     2821 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     2881 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     2941 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     3001 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     3061 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     3121 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     3181 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     3241 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3301 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgacccg
     3361 ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     3421 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     3481 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     3541 ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
     3601 tcgataagga tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt
     3661 aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     3721 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     3781 agcgagcgag cgcgcagc
//
