LOCUS       62056_216        6239 bp DNA     circular SYN 17-NOV-2020
DEFINITION  Cloning vector AAV-CaMKII-mRuby2-4X128-mAGNET, complete sequence.
ACCESSION   MW139899
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Keaveney MK, Rahsepar B, Tseng HA, Fernandez FR, Mount RA, Ta T, 
            White JA, Berg J, Han X.
  TITLE     CamKIIalpha Positive Interneurons Identified via A microRNA Based 
            Viral Gene Targeting Strategy
  JOURNAL   J Neurosci (2020) In press
  PUBMED    33158963
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Keaveney MK, Rahsepar B, Tseng HA, Fernandez FR, Mount RA, Ta T, 
            White JA, Berg J, Han X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) Biomedical Engineering, Boston University, 
            44 Cummington Mall, Boston, MA 02215, USA
REFERENCE   3  (bases 1 to 6239)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-OCT-2020) Biomedical Engineering, Boston University, 44 
            Cummington Mall, Boston, MA 02215, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    228..249
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        264..294
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    302..318
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     326..342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   380..480
     promoter        576..1868
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             1888..2598
                     /label=mRuby2
                     /note="monomeric red fluorescent protein derived from
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
     misc_binding    2616..2636
                     /label=miRNA-128 binding site
                     /bound_moiety="miRNA-128"
     misc_binding    2642..2662
                     /label=miRNA-128 binding site
                     /bound_moiety="miRNA-128"
     misc_binding    2668..2688
                     /label=miRNA-128 binding site
                     /bound_moiety="miRNA-128"
     misc_binding    2694..2714
                     /label=miRNA-128 binding site
                     /bound_moiety="miRNA-128"
     misc_feature    2748..3336
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(3339..3355)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(3385..3506)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3609..3798
     primer_bind     complement(3816..3832)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3974..4429
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4455..4559
                     /label=AmpR promoter
     CDS             4560..5417
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5591..6179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt
      361 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      421 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      481 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      541 atctacgtag ccatgctcta ggaagatctg aattccatta tggccttagg taggtcactt
      601 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt
      661 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt
      721 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact
      781 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca
      841 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc
      901 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat
      961 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc
     1021 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat
     1081 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct
     1141 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc
     1201 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga
     1261 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac
     1321 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc
     1381 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa
     1441 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat
     1501 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc
     1561 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa
     1621 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt
     1681 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga
     1741 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca
     1801 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg
     1861 ggggcagcgg ggcgcgccgc cgccaccatg gtgtctaagg gcgaagagct gatcaaggaa
     1921 aatatgcgta tgaaggtggt catggaaggt tcggtcaacg gccaccaatt caaatgcaca
     1981 ggtgaaggag aaggcaatcc gtacatggga actcaaacca tgaggatcaa agtcatcgag
     2041 ggaggacccc tgccatttgc ctttgacatt cttgccacgt cgttcatgta tggcagccgt
     2101 acttttatca agtacccgaa aggcattcct gatttcttta aacagtcctt tcctgagggt
     2161 tttacttggg aaagagttac gagatacgaa gatggtggag tcgtcaccgt catgcaggac
     2221 accagccttg aggatggctg tctcgtttac cacgtccaag tcagaggggt aaactttccc
     2281 tccaatggtc ccgtgatgca gaagaagacc aagggttggg agcctaatac agagatgatg
     2341 tatccagcag atggtggtct gaggggatac actcatatgg cactgaaagt tgatggtggt
     2401 ggccatctgt cttgctcttt cgtaacaact tacaggtcaa aaaagaccgt cgggaacatc
     2461 aagatgcccg gtatccatgc cgttgatcac cgcctggaaa ggttagagga aagtgacaat
     2521 gaaatgttcg tagtacaacg cgaacacgca gttgccaagt tcgccgggct tggtggtggg
     2581 atggacgagc tgtacaagta aactagtgcg gccttaaaga gaccggttca ctgtgacagt
     2641 aaaagagacc ggttcactgt gagaatgaaa gagaccggtt cactgtgatc ggaaaagaga
     2701 ccggttcact gtgagcggcc gccctgcagg catgcaagct tatcgataat caacctctgg
     2761 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     2821 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     2881 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     2941 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     3001 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     3061 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     3121 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     3181 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     3241 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3301 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgacccg
     3361 ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     3421 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     3481 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     3541 ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
     3601 tcgataagga tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt
     3661 aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     3721 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     3781 agcgagcgag cgcgcagcct taattaacct aattcactgg ccgtcgtttt acaacgtcgt
     3841 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3901 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3961 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4021 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4081 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4141 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4201 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4261 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4321 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4381 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     4441 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4501 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4561 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4621 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4681 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4741 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4801 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4861 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4921 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4981 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5041 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5101 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5161 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5221 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5281 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5341 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5401 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5461 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5521 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5581 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5641 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5701 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5761 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5821 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5881 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5941 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6001 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6061 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6121 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6181 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatg
//
