LOCUS       62056_156        6727 bp DNA     circular SYN 19-MAR-2020
DEFINITION  Cloning vector 5-PLTP:ODP2:OS-T28, complete sequence.
ACCESSION   MN294717
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Gao H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2019) Molecular Engineering in AST, Corteva 
            Agriscience, 8305 NW 62nd Ave., Johnston, IA 50131, USA
REFERENCE   2  (bases 1 to 6727)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (08-AUG-2019) Molecular Engineering in AST, Corteva Agriscience, 
            8305 NW 62nd Ave., Johnston, IA 50131, USA"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. 10
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     51..67
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    83..182
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      221..1191
                     /regulatory_class="promoter"
     CDS             1285..3417
                     /codon_start=1
                     /transl_table=11
                     /product="ZM-ODP2"
                     /label=ZM-ODP2
                     /protein_id="QIN53503.1"
                     /translation="MATVNNWLAFSLSPQELPPSQTTDSTLISAATADHVSGDVCFNIP
                     QDWSMRGSELSALVAEPKLEDFLGGISFSEQHHKANCNMIPSTSSTVCYASSGASTGYH
                     HQLYHQPTSSALHFADSVMVASSAGVHDGGAMLSAAAANGVAGAASANGGGIGLSMIKN
                     WLRSQPAPMQPRVAAAEGAQGLSLSMNMAGTTQGAAGMPLLAGERARAPESVSTSAQGG
                     AVVVTAPKEDSGGSGVAGALVAVSTDTGGSGGASADNTARKTVDTFGQRTSIYRGVTRH
                     RWTGRYEAHLWDNSCRREGQTRKGRQVYLGGYDKEEKAARAYDLAALKYWGATTTTNFP
                     VSNYEKELEDMKHMTRQEFVASLRRKSSGFSRGASIYRGVTRHHQHGRWQARIGRVAGN
                     KDLYLGTFSTQEEAAEAYDIAAIKFRGLNAVTNFDMSRYDVKSILDSSALPIGSAAKRL
                     KEAEAAASAQHHHAGVVSYDVGRIASQLGDGGALAAAYGAHYHGAAWPTIAFQPGAAST
                     GLYHPYAQQPMRGGGWCKQEQDHAVIAAAHSLQDLHHLNLGAAGAHDFFSAGQQAAAAA
                     MHGLGSIDSASLEHSTGSNSVVYNGGVGDSNGASAVGGSGGGYMMPMSAAGATTTSAMV
                     SHEQVHARAYDEAKQAAQMGYESYLVNAENNGGGRMSAWGTVVSAAAAAAASSNDNMAA
                     DVGHGGAQLFSVWNDT"
     regulatory      3435..4315
                     /regulatory_class="terminator"
     protein_bind    complement(4366..4465)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(4483..4501)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4506..4522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4635..5441
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      5591..6179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(6509..6536)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(6628..6714)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac
       61 ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag tgacctgttc
      121 gttgcaacaa attgatgagc aatgcttttt tataatgcca agtttgtaca aaaaagcagg
      181 ctccggccag aatggcccgg accgggttac cgaattctta ccctagctcc ctgcggctgt
      241 tacgcggtcc cccatcaatc ttctgttctt gcggttgtag cctgtgtaac agtgctagag
      301 tatgtatgat aaataggttt taagtctgct tacatgacat tttttattgt ggaagagaca
      361 tataaaaatt agagagagtg gttctcatgc aacggcggac ggcccggtgc taaaagagct
      421 tcaagacaaa ataatgaaac aggaagagag tagatttatc taagagccaa ctttattata
      481 tgaatgtgtt tattgttggc tttagatgat atggtaagga gttagagcta ataatagata
      541 ggctctatta ttattattat taattaaact cgctctaagg aggaaagtgg gaggaaggga
      601 cgaggacgaa gactactgga agcatcgtgc atggatgatg gatgtggtgt ctcttaatgt
      661 aggtggccgg aggatgtacg tgttaattgc gcgataagca ctcagatcca accgcaaact
      721 acctccacac tgacacactg atagagagaa agagagacct ccgacgactg ccgccgcaga
      781 tgagccacgt acgtatacga cgtctgccgg ccggctcagg ctgccgccat caccctgctc
      841 gaaagtcgcg ttaggcggcg ccagctacat aggagtatct agtctagcca gttagtatac
      901 tactactgcg ctgatgatga attaactctg catagatact gtacttgcct ccctccaaca
      961 cccaaccacc tcctgctcgg ctcttaataa cttggacacg gatcgatgcc atccaaggaa
     1021 gaacacgacg acgacgacgg aacatccacc atgcaagctt gcatccatac gccgatacgc
     1081 gtgcatccat ccatccacca ttatttccat tttccaccga tcacacgtac acaggcctat
     1141 ttaaggagcg acatcccact gcaactctcc tcaccactca tcaccagcta gctctagcaa
     1201 agcacttgcc atctaccgac cgccgcattc caaacagccc gacgagctag cagagcggca
     1261 ggcacctccc tcctcaagga acccatggcc actgtgaaca actggctcgc tttctccctc
     1321 tccccgcagg agctgccgcc ctcccagacg acggactcca cactcatctc ggccgccacc
     1381 gccgaccatg tctccggcga tgtctgcttc aacatccccc aagattggag catgagggga
     1441 tcagagcttt cggcgctcgt cgcggagccg aagctggagg acttcctcgg cggcatctcc
     1501 ttctccgagc agcatcacaa ggccaactgc aacatgatac ccagcactag cagcacagtt
     1561 tgctacgcga gctcaggtgc tagcaccggc taccatcacc agctgtacca ccagcccacc
     1621 agctcagcgc tccacttcgc ggactccgta atggtggctt cctcggccgg tgtccacgac
     1681 ggcggtgcca tgctcagcgc ggccgccgct aacggtgtcg ctggcgctgc cagtgccaac
     1741 ggcggcggca tcgggctgtc catgattaag aactggctgc ggagccaacc ggcgcccatg
     1801 cagccgaggg tggcggcggc tgagggcgcg caggggctct ctttgtccat gaacatggcg
     1861 gggacgaccc aaggcgctgc tggcatgcca cttctcgctg gagagcgcgc acgggcgccc
     1921 gagagtgtat cgacgtcagc acagggtgga gccgtcgtcg tcacggcgcc gaaggaggat
     1981 agcggtggca gcggtgttgc cggcgctcta gtagccgtga gcacggacac gggtggcagc
     2041 ggcggcgcgt cggctgacaa cacggcaagg aagacggtgg acacgttcgg gcagcgcacg
     2101 tcgatttacc gtggcgtgac aaggcataga tggactggga gatatgaggc acatctttgg
     2161 gataacagtt gcagaaggga agggcaaact cgtaagggtc gtcaagtcta tttaggtggc
     2221 tatgataaag aggagaaagc tgctagggct tatgatcttg ctgctctgaa gtactggggt
     2281 gccacaacaa caacaaattt tccagtgagt aactacgaaa aggagctcga ggacatgaag
     2341 cacatgacaa ggcaggagtt tgtagcgtct ctgagaagga agagcagtgg tttctccaga
     2401 ggtgcatcca tttacagggg agtgactagg catcaccaac atggaagatg gcaagcacgg
     2461 attggacgag ttgcagggaa caaggatctt tacttgggca ccttcagcac ccaggaggag
     2521 gcagcggagg cgtacgacat cgcggcgatc aagttccgcg gcctcaacgc cgtcaccaac
     2581 ttcgacatga gccgctacga cgtgaagagc atcctggaca gcagcgccct ccccatcggc
     2641 agcgccgcca agcgcctcaa ggaggccgag gccgcagcgt ccgcgcagca ccaccacgcc
     2701 ggcgtggtga gctacgacgt cggccgcatc gcctcgcagc tcggcgacgg cggagccctg
     2761 gcggcggcgt acggcgcgca ctaccacggc gccgcctggc cgaccatcgc gttccagccg
     2821 ggcgccgcca gcacaggcct gtaccacccg tacgcgcagc agccaatgcg cggcggcggg
     2881 tggtgcaagc aggagcagga ccacgcggtg atcgcggccg cgcacagcct gcaggacctc
     2941 caccacctga acctgggcgc ggccggcgcg cacgactttt tctcggcagg gcagcaggcc
     3001 gccgccgctg cgatgcacgg cctgggtagc atcgacagtg cgtcgctcga gcacagcacc
     3061 ggctccaact ccgtcgtcta caacggcggg gtcggcgaca gcaacggcgc cagcgccgtc
     3121 ggcggcagtg gcggtggcta catgatgccg atgagcgctg ccggagcaac cactacatcg
     3181 gcaatggtga gccacgagca ggtgcatgca cgggcctacg acgaagccaa gcaggctgct
     3241 cagatggggt acgagagcta cctggtgaac gcggagaaca atggtggcgg aaggatgtct
     3301 gcatggggga ctgtcgtgtc tgcagccgcg gcggcagcag caagcagcaa cgacaacatg
     3361 gccgccgacg tcgggcatgg cggcgcgcag ctcttcagtg tctggaacga cacttaagcg
     3421 tacctagtgg tacctgacat cttatagtct gcaacctctc gtgtctgaat tcctatcttt
     3481 atcaagtgtt attgcttcca cgactatagg acagctttcg tcgaaagctt ttgctcatgt
     3541 gatctcgaag gattcatcta gtctgatttt tcgtgacttg tatcggtttt attggattca
     3601 tccaacatat atcaataaaa aatgagttgt gtttcctttc ttcctagttc agttaaaatt
     3661 atttccctcc tgcgcttgtg ctgtaattgt ctgtgtacct gttgtttgtg actgtgttag
     3721 ttcccttgga tatgatttcg tatttgatat gtacatggag atagcttagc ttcattattg
     3781 gagtatgaag ttagtatgac atagtcactc tcctggaaaa ttgacactgc aaaccatatt
     3841 tttattctga accacaaatc ctagtcagtc cgctggcata tgccgtccgt ttgctgaatc
     3901 cagaacgtgg gtttggagat gtacggctga gatgcctcta tgcgaagggg atttcgtggt
     3961 gaaacgagat gggagtagag caacgcccgt ggaagatgct tcaaacttcc acacttttga
     4021 gcaacgatcg gcagtagtaa ggtagacgat ttcaagatca aagcatatga agataaacaa
     4081 catcaacaac aaaatttgtt ggggttctat agagagaaac agagctacat acatacactg
     4141 ttttgtatct accatctgag atgatgaaaa gatgaaaaac taaagaatgc cccggcgcca
     4201 acgccaggac acgccgcgcg cgcgtcaccc gagccatctc ttgacccagc cggcgctgta
     4261 tatttacaca cgttgcagca tcgatcacca cctgttcgat cgcgtcgccg tcaccggtac
     4321 cgagctcgaa ttccggtccg ggcctagaag gccgatctcc cgggcaccca gctttcttgt
     4381 acaaagtggg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     4441 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     4501 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     4561 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     4621 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     4681 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     4741 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     4801 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     4861 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     4921 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     4981 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     5041 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     5101 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     5161 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     5221 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     5281 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     5341 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     5401 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     5461 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     5521 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     5581 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5641 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5701 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     5761 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5821 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5881 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5941 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     6001 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6061 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6121 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6181 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6241 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6301 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6361 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     6421 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     6481 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     6541 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     6601 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     6661 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     6721 ggcagtt
//
