LOCUS       40924_90        5715 bp DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Saxena MT, Schroeter EH, Mumm JS, Kopan R
  TITLE     Murine notch homologs (N1-4) undergo presenilin-dependent 
            proteolysis.
  JOURNAL   J Biol Chem. 2001 Oct 26;276(43):40268-73. Epub 2001 Aug 22.
  PUBMED    11518718
REFERENCE   2  (bases 1 to 5715)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5715)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol
            Chem."; date: "2001-10-26"; volume: "276(43)"; pages: "40268-73.
            Epub 2001 Aug 22"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    86..207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             425..2074
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     intron          2318..2383
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2513..2533
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    2958..3092
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3234..3251)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3405..3993)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4167..5024)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5025..5129)
                     /label=AmpR promoter
     rep_origin      5156..5611
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtacgtggag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
       61 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
      121 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
      181 tttgtccaaa ctcatcaatg tatcttatgg tactgtaact gagctaacat aacccgggga
      241 attcgccgtg ggaaaatcgc cgtgggaaaa tcgccgtggg aaaatcgccg tgggaaaatt
      301 cgaggatctc cgcattgcag agataattgt atttaagtgc ctagctcgat acaataaacg
      361 ccatttgacc attcaccaca ttggtgtgca cctccaagct tggcattccg gtactgttgg
      421 taaaatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc ctctagagga
      481 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac
      541 aattgctttt acagatgcac atatcgaggt gaacatcacg tacgcggaat acttcgaaat
      601 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt
      661 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg
      721 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatgaa
      781 catttcgcag cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt
      841 gcaaaaaaaa ttaccaataa tccagaaaat tattatcatg gattctaaaa cggattacca
      901 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata
      961 cgattttgta ccagagtcct ttgatcgtga caaaacaatt gcactgataa tgaattcctc
     1021 tggatctact gggttaccta agggtgtggc ccttccgcat agaactgcct gcgtcagatt
     1081 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag
     1141 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg
     1201 atttcgagtc gtcttaatgt atagatttga agaagagctg tttttacgat cccttcagga
     1261 ttacaaaatt caaagtgcgt tgctagtacc aaccctattt tcattcttcg ccaaaagcac
     1321 tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctgggg gcgcacctct
     1381 ttcgaaagaa gtcggggaag cggttgcaaa acgcttccat cttccaggga tacgacaagg
     1441 atatgggctc actgagacta catcagctat tctgattaca cccgaggggg atgataaacc
     1501 gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg
     1561 gaaaacgctg ggcgttaatc agagaggcga attatgtgtc agaggaccta tgattatgtc
     1621 cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca
     1681 ttctggagac atagcttact gggacgaaga cgaacacttc ttcatagttg accgcttgaa
     1741 gtctttaatt aaatacaaag gatatcaggt ggcccccgct gaattggaat cgatattgtt
     1801 acaacacccc aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg acgccggtga
     1861 acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt
     1921 ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt
     1981 ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct
     2041 cataaaggcc aagaagggcg gaaagtccaa attgtaaaat gtaactgtat tcagcgatga
     2101 cgaaattctt agctattgta atactgcgat gagtggcagg gcggggcgta atttttttaa
     2161 ggcagttatt ggtgccctta aacgcctggt tgctacgcct gaataagtga taataagcgg
     2221 atgaatggca gaaattcgcc ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     2281 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     2341 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     2401 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     2461 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     2521 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     2581 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     2641 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
     2701 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
     2761 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
     2821 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     2881 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     2941 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3001 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3061 aactcatcaa tgtatcttat catgtctgga tccgtcgacc gatgcccttg agagccttca
     3121 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     3181 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
     3241 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3301 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3361 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3421 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3481 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3541 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3601 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3661 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3721 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3781 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3841 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3901 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3961 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4021 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4081 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4141 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4201 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4261 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4321 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4381 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4441 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4501 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4561 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4621 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4681 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4741 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     4801 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4861 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4921 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4981 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5041 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5101 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     5161 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5221 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5281 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5341 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5401 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5461 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5521 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5581 aattttaaca aaatattaac gcttacaatt tgccattcgc cattcaggct gcgcaactgt
     5641 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct accatgataa
     5701 gtaagtaata ttaag
//
