LOCUS       62056_146        5780 bp DNA     circular SYN 19-MAR-2020
DEFINITION  Cloning vector 4-UBI:NPTII:PINII, complete sequence.
ACCESSION   MN294716
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Gao H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2019) Molecular Engineering in AST, Corteva 
            Agriscience, 8305 NW 62nd Ave., Johnston, IA 50131, USA
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (08-AUG-2019) Molecular Engineering in AST, Corteva Agriscience, 
            8305 NW 62nd Ave., Johnston, IA 50131, USA"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. 10
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(256..355)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(373..391)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(396..412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             525..1331
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1481..2069
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(2399..2426)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(2518..2604)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     2668..2684
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2700..2799
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2834..4825
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             4850..5638
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="VEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 ctagttatct gaataaaaga gaaagagatc atccatattt cttatcctaa atgaatgtca
       61 cgtgtcttta taattctttg atgaaccaga tgcatttcat taaccaaatc catatacata
      121 taaatattaa tcatatataa ttaatatcaa ttgggttagc aaaacaaatc tagtctaggt
      181 gtgttttgcg aatgcggccg ccaccgcggt ggagctcgaa ttccggtccg ggcctagaag
      241 gccgatctcc cgggcaccca gctttcttgt acaaagtggg cattataaga aagcattgct
      301 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
      361 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
      421 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
      481 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
      541 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
      601 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
      661 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
      721 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
      781 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc
      841 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
      901 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
      961 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     1021 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     1081 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     1141 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     1201 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     1261 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     1321 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     1381 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     1441 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1501 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1561 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1621 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1681 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1741 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1801 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1861 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1921 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1981 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2041 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2101 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2161 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2221 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     2281 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     2341 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
     2401 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     2461 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     2521 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     2581 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag
     2641 catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc
     2701 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat
     2761 gcttttttat aatgccaagt ttgtacaaaa aagcaggctc cggccagaat ggcccggacc
     2821 gaagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc ccctctctag agataatgag
     2881 cattgcatgt ctaagttata aaaaattacc acatattttt tttgtcacac ttgtttgaag
     2941 tgcagtttat ctatctttat acatatattt aaactttact ctacgaataa tataatctat
     3001 agtactacaa taatatcagt gttttagaga atcatataaa tgaacagtta gacatggtct
     3061 aaaggacaat tgagtatttt gacaacagga ctctacagtt ttatcttttt agtgtgcatg
     3121 tgttctcctt tttttttgca aatagcttca cctatataat acttcatcca ttttattagt
     3181 acatccattt agggtttagg gttaatggtt tttatagact aattttttta gtacatctat
     3241 tttattctat tttagcctct aaattaagaa aactaaaact ctattttagt ttttttattt
     3301 aataatttag atataaaata gaataaaata aagtgactaa aaattaaaca aatacccttt
     3361 aagaaattaa aaaaactaag gaaacatttt tcttgtttcg agtagataat gccagcctgt
     3421 taaacgccgt cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc
     3481 caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc
     3541 gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga
     3601 cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcacggcagc tacgggggat
     3661 tcctttccca ccgctccttc gctttccctt cctcgcccgc cgtaataaat agacaccccc
     3721 tccacaccct ctttccccaa cctcgtgttg ttcggagcgc acacacacac aaccagatct
     3781 cccccaaatc cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcc tccccccccc
     3841 cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac
     3901 ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta
     3961 cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt
     4021 ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt
     4081 gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt
     4141 gtttgtcggg tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt
     4201 gggcggtcgt tctagatcgg agtagaattc tgtttcaaac tacctggtgg atttattaat
     4261 tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg
     4321 aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag
     4381 atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc
     4441 tagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta
     4501 tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg
     4561 ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat
     4621 ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa
     4681 ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt
     4741 tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc
     4801 ctgttgtttg gtgttacttc tgcaggtcga ctctagagga tccaccatgg ttgaacaaga
     4861 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     4921 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4981 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     5041 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     5101 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     5161 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     5221 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     5281 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     5341 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg atgatctcgt
     5401 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     5461 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     5521 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     5581 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5641 aggatccacc atggttaacc tagacttgtc catcttctgg attggccaac ttaattaatg
     5701 tatgaaataa aaggatgcac acatagtgac atgctaatca ctataatgtg ggcatcaaag
     5761 ttgtgtgtta tgtgtaatta
//
