LOCUS       40924_45        3792 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector 35SCore-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Grant TN, De La Torre CM, Zhang N, Finer JJ.
  TITLE     Synthetic introns help identify sequences in the 5' UTR intron of 
            the Glycine max polyubiquitin (Gmubi) promoter that give increased 
            promoter activity
  JOURNAL   Planta (2017) In press
  PUBMED    28070655
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Finer J, Grant T, De La Torre C, Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 3792)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3792)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Planta 
            (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-SEP-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        447..493
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             512..1228
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     terminator      1264..1516
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1571..1587)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1595..1611)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1619..1649)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1664..1685)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1973..2561)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2735..3592)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3593..3697)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctgcag ctagcggtac
      421 cactagtccc gggtctagag tcgaccgcaa gacccttcct ctatataagg aagttcattt
      481 catttggaga ggacacgctc gagtggccac catggtgagc aagggcgagg agctgttcac
      541 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      601 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      661 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcacct acggcgtgca
      721 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      781 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      841 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      901 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
      961 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     1021 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1081 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     1141 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1201 cactcacggc atggacgagc tgtacaagta aagcggccgc ccggctgcac tgaggcctca
     1261 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     1321 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     1381 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     1441 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     1501 tctatgttac tagatccgat gataagctgt caaacatgag aattagatct caattgaatt
     1561 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1621 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1681 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     1741 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1801 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1861 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1921 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1981 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2041 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2101 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2161 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2221 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2281 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2341 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2401 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2461 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2521 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2581 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2641 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2701 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2761 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2821 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2881 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2941 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3001 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3061 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3121 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3181 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3241 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3301 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3361 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3421 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3481 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3541 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3601 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3661 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3721 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3781 ggccctttcg tc
//
