LOCUS       62056_111        5077 bp DNA     circular SYN 20-JAN-2020
DEFINITION  Cloning vector 35S-LUC, complete sequence.
ACCESSION   MN728542
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2019) State Key Laboratory of Plant Genomics, 
            Institute of Genetics and Developmental Biology, CAS, West Beichen 
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (22-NOV-2019) State Key Laboratory of Plant Genomics, Institute of 
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..5077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        104..150
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             244..1893
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     terminator      1914..2166
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2175..2191)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2665..2769
                     /label=AmpR promoter
     CDS             2770..3627
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3801..4389
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4677..4698
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4713..4743
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4751..4767
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4775..4791
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 actccgctcg gaggacagta ctccgctcgg aggacagtac tccgctcgag gacagtactc
       61 cgctcggagg acagtactcc gatccgtcga ctctagagga tctgcaagac ccttcctcta
      121 tataaggaag ttcatttcat ttggagagga cacgctggat ccacaattac caacaacaac
      181 aaacaacaaa caacattaca attacagatc cattacaatt actatttaca attacagtcg
      241 accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat
      301 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca
      361 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg
      421 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc
      481 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga
      541 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc
      601 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg
      661 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag
      721 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac
      781 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct
      841 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc
      901 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt
      961 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga
     1021 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat
     1081 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact
     1141 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc
     1201 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga
     1261 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg
     1321 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg
     1381 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc
     1441 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat
     1501 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag
     1561 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc
     1621 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa
     1681 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg
     1741 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg
     1801 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc
     1861 ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agcgaatttc cccgatcgtt
     1921 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta
     1981 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
     2041 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
     2101 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
     2161 tagatcggga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     2221 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     2281 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     2341 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     2401 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     2461 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     2521 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     2581 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     2641 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     2701 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     2761 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     2821 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     2881 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     2941 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     3001 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     3061 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     3121 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     3181 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     3241 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     3301 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     3361 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     3421 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     3481 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     3541 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     3601 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     3661 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     3721 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     3781 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3841 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3901 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3961 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4021 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4081 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4141 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4201 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4261 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4321 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4381 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4441 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4501 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     4561 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     4621 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4681 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     4741 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     4801 gccaagcttg catgcctgca ggtccccaga ttagcctttt caatttcaga aagaatgcta
     4861 acccacagat ggttagagag gcttacgcag caggtctcat caagacgatc tacccgagca
     4921 ataatctcca ggaaatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca
     4981 ggactaactg catcaagaac acagagaaag atatatttct caagatcaga agtactattc
     5041 cagtatggac gattcaaggc ttgcttcaca aaccaag
//
