LOCUS       62056_106        4696 bp DNA     circular SYN 20-JAN-2020
DEFINITION  Cloning vector 35S-GFP, complete sequence.
ACCESSION   MN728541
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2019) State Key Laboratory of Plant Genomics, 
            Institute of Genetics and Developmental Biology, CAS, West Beichen 
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (22-NOV-2019) State Key Laboratory of Plant Genomics, Institute of 
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..4696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      89..341
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(359..375)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(383..399)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(407..437)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(452..473)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(761..1349)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1523..2380)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2381..2485)
                     /label=AmpR promoter
     primer_bind     2959..2975
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3489..3834
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3918..4634
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
       61 ctgtacaagt aagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
      121 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
      181 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
      241 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
      301 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattcg taatcatggt
      361 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      421 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      481 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      541 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      601 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      661 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      721 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      781 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      841 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      901 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      961 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1021 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1081 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1141 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1201 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1261 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1321 agattacgcg cagaagaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1381 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1441 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1501 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1561 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1621 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1681 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1741 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1801 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1861 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1921 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1981 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2041 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2101 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2161 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2221 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2281 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     2341 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     2401 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     2461 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     2521 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     2581 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     2641 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     2701 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     2761 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     2821 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     2881 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     2941 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcc
     3001 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3061 cgcagcaggt ctcatcaaga gatctacccg agcaataatc tccaggaaat caaatacctt
     3121 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
     3181 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     3241 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     3301 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     3361 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     3421 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     3481 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     3541 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     3601 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     3661 gatggccccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     3721 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     3781 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     3841 actctagagt taaccgggct caggcctggc gcgccactag tggatccatc gatagtactg
     3901 tcgacctcga gggtaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3961 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     4021 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4081 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     4141 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4201 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     4261 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     4321 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     4381 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     4441 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4501 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     4561 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4621 acgagctgta caagtaagag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt
     4681 ttcttaagat tgaatc
//
