LOCUS       62056_96        3700 bp DNA     circular SYN 19-MAR-2020
DEFINITION  Cloning vector 3-waxy-guide-CR3, complete sequence.
ACCESSION   MN294715
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Gao H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2019) Molecular Engineering in AST, Corteva 
            Agriscience, 8305 NW 62nd Ave., Johnston, IA 50131, USA
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (08-AUG-2019) Molecular Engineering in AST, Corteva Agriscience, 
            8305 NW 62nd Ave., Johnston, IA 50131, USA"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. 10
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    15..114
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      198..1197
                     /regulatory_class="promoter"
     misc_RNA        1217..1292
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(1364..1459)
                     /label=attL3
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1501..1519)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1524..1540)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1653..2459
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2609..3197
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(3527..3554)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
       61 aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca ggctccggcc
      121 agaatggccc ggaccgggtt accgaattcg agctcggtac cctgggatcc gatatcgatg
      181 ggccctggcc gaagctttga gagtacaatg atgaacctag attaatcaat gccaaagtct
      241 gaaaaatgca ccctcagtct atgatccaga aaatcaagat tgcttgaggc cctgttcggt
      301 tgttccggat tagagccccg gattaattcc tagccggatt acttctctaa tttatataga
      361 ttttgatgag ctggaatgaa tcctggctta ttccggtaca accgaacagg ccctgaagga
      421 taccagtaat cgctgagcta aattggcatg ctgtcagagt gtcagtattg cagcaaggta
      481 gtgagataac cggcatcatg gtgccagttt gatggcacca ttagggttag agatggtggc
      541 catgggcgca tgtcctggcc aactttgtat gatatatggc agggtgaata ggaaagtaaa
      601 attgtattgt aaaaagggat ttcttctgtt tgttagcgca tgtacaagga atgcaagttt
      661 tgagcgaggg ggcatcaaag atctggctgt gtttccagct gtttttgtta gccccatcga
      721 atccttgaca taatgatccc gcttaaataa gcaacctcgc ttgtatagtt ccttgtgctc
      781 taacacacga tgatgataag tcgtaaaata gtggtgtcca aagaatttcc aggcccagtt
      841 gtaaaagcta aaatgctatt cgaatttcta ctagcagtaa gtcgtgttta gaaattattt
      901 ttttatatac cttttttcct tctatgtaca gtaggacaca gtgtcagcgc cgcgttgacg
      961 gagaatattt gcaaaaaagt aaaagagaaa gtcatagcgg cgtatgtgcc aaaaacttcg
     1021 tcacagagag ggccataaga aacatggccc acggcccaat acgaagcacc gcgacgaagc
     1081 ccaaacagca gtccgtaggt ggagcaaagc gctgggtaat acgcaaacgt tttgtcccac
     1141 cttgactaat cacaagagtg gagcgtacct tataaaccga gccgcaagca ccgaattgca
     1201 atataaaatt accgatgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
     1261 atcaacttga aaaagtggca ccgagtcggt gctttttttt tgcggccgcg aattcctgca
     1321 gggccctctt gtcggaccag ttgcccacca cgttggtgag gggcaacttt attatacaaa
     1381 gttggcatta taaaaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1441 caaaataaaa tcattatttg gagctccatg catggtagcg ttaacgcggc cgcgatatcc
     1501 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     1561 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     1621 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
     1681 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     1741 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
     1801 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     1861 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     1921 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
     1981 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     2041 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     2101 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     2161 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     2221 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     2281 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     2341 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     2401 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     2461 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     2521 gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     2581 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2641 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2701 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2761 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2821 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2881 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2941 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3001 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3061 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3121 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3181 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3241 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3301 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3361 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3421 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3481 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     3541 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     3601 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     3661 gagagcgttc accgacaaac aacagataaa acgaaaggcc
//
