LOCUS       62056_81        3701 bp DNA     circular SYN 19-MAR-2020
DEFINITION  Cloning vector 2-waxy-guide-CR1, complete sequence.
ACCESSION   MN294714
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Gao H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2019) Molecular Engineering in AST, Corteva 
            Agriscience, 8305 NW 62nd Ave., Johnston, IA 50131, USA
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (08-AUG-2019) Molecular Engineering in AST, Corteva Agriscience, 
            8305 NW 62nd Ave., Johnston, IA 50131, USA"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. 10
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    6..105
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      189..1188
                     /regulatory_class="promoter"
     misc_RNA        1209..1284
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(1356..1451)
                     /label=attL3
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1493..1511)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1516..1532)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1645..2451
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2601..3189
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(3519..3546)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg
       61 agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggctccggc cagaatggcc
      121 cggaccgggt taccgaattc gagctcggta ccctgggatc cgatatcgat gggccctggc
      181 cgaagctttg agagtacaat gatgaaccta gattaatcaa tgccaaagtc tgaaaaatgc
      241 accctcagtc tatgatccag aaaatcaaga ttgcttgagg ccctgttcgg ttgttccgga
      301 ttagagcccc ggattaattc ctagccggat tacttctcta atttatatag attttgatga
      361 gctggaatga atcctggctt attccggtac aaccgaacag gccctgaagg ataccagtaa
      421 tcgctgagct aaattggcat gctgtcagag tgtcagtatt gcagcaaggt agtgagataa
      481 ccggcatcat ggtgccagtt tgatggcacc attagggtta gagatggtgg ccatgggcgc
      541 atgtcctggc caactttgta tgatatatgg cagggtgaat aggaaagtaa aattgtattg
      601 taaaaaggga tttcttctgt ttgttagcgc atgtacaagg aatgcaagtt ttgagcgagg
      661 gggcatcaaa gatctggctg tgtttccagc tgtttttgtt agccccatcg aatccttgac
      721 ataatgatcc cgcttaaata agcaacctcg cttgtatagt tccttgtgct ctaacacacg
      781 atgatgataa gtcgtaaaat agtggtgtcc aaagaatttc caggcccagt tgtaaaagct
      841 aaaatgctat tcgaatttct actagcagta agtcgtgttt agaaattatt tttttatata
      901 ccttttttcc ttctatgtac agtaggacac agtgtcagcg ccgcgttgac ggagaatatt
      961 tgcaaaaaag taaaagagaa agtcatagcg gcgtatgtgc caaaaacttc gtcacagaga
     1021 gggccataag aaacatggcc cacggcccaa tacgaagcac cgcgacgaag cccaaacagc
     1081 agtccgtagg tggagcaaag cgctgggtaa tacgcaaacg ttttgtccca ccttgactaa
     1141 tcacaagagt ggagcgtacc ttataaaccg agccgcaagc accgaattgc ccaaaacgct
     1201 ttcccgatgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
     1261 gaaaaagtgg caccgagtcg gtgctttttt tttgcggccg cgaattcctg cagggccctc
     1321 ttgtcggacc agttgcccac cacgttggtg aggggcaact ttattataca aagttggcat
     1381 tataaaaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     1441 aatcattatt tggagctcca tgcatggtag cgttaacgcg gccgcgatat cccctatagt
     1501 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     1561 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     1621 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     1681 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     1741 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     1801 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     1861 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     1921 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     1981 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     2041 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     2101 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     2161 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     2221 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     2281 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2341 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2401 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     2461 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     2521 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     2581 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2641 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2701 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2761 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2821 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2881 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2941 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3001 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3061 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3121 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3181 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3241 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3301 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3361 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3421 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     3481 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     3541 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     3601 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     3661 tcaccgacaa acaacagata aaacgaaagg ccttaagctc g
//
