Rat TNFR1/CD120a/TNFRSF1A (Fc Tag) recombinant protein

Rat TNFR1/CD120a/TNFRSF1A (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis (By similarity).

Tumor necrosis factor receptor superfamily member 1A

TNFR1; P60; TNFR55; TNFRSF1A; TNF-R; TNF-R55; P55-R; TNFAR; TBP1; TNFR60; TNF-R-I; P55; CD120A; MGC19588; FPF

P22934

Tnfrsf1a; Tnfr-1; Tnfr1

Human Cells

A DNA sequence encoding the rat TNFRSF1A (P22934) (Met1-Ala211) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

Rat

The recombinant rat TNFRSF1A/Fc is a disulfide-linked homodimer. The reduced monomer comprises 431 amino acids and has a predicted molecular mass of 47.9 kDa. The apparent molecular mass of the protein is approximately 59 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

1. Measured by its ability to inhibit the TNFα mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is typically 3-30 ng/mL in the presence of 0.05 ng/mL of ratTNFα.
2. Immobilized mouse TNFa (80-235) (Cat:500439 ) at 10 μg/ml (100 μl/well) can bind rat TNFRSF1A-Fc, The EC₅₀ of rat TNFRSF1A-Fc is 0.08-0.20 μg/ml.