Human Osteoprotegerin/TNFRSF11B (His Tag) recombinant protein

Human Osteoprotegerin/TNFRSF11B (His Tag) recombinant protein

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• Material Safety Data Sheets (MSDS)

Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.

Tumor necrosis factor receptor superfamily member 11B

OSTEOPROTEGERIN; TR1; TNFRSF11B; OCIF; OPG; MGC29565

O00300

TNFRSF11B; OCIF; OPG

Human Cells

A DNA sequence encoding the human TNFRSF11B (NP_002537.3) (Met 1-Leu 401) was fused with a polyhistidine tag at the C-terminus.

Human

The secreted recombinant human TNFRSF11B comprises 391 amino acids and has a predicted molecular mass of 45.3 kDa. As a result of glycosylation, rhTNFRSF11B migrates as an approximately 55 kDa bnd in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

1. Measured by its ability to inhibit TRAIL mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED₅₀ for this effect is typically 5-20 ng/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10 (Catalog # 10409-HNAE).
2. Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF11B-His at 10 μg/ml (100 μl/well) can bind human Fc-TNFSF11 (Cat：11682-H01H) with a linear ranger of 3.125-200 ng/mL.