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  • Human Osteoprotegerin/TNFRSF11B (His Tag) recombinant protein

Human Osteoprotegerin/TNFRSF11B (His Tag) recombinant protein

Cat.#: 500115

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Product Information

  • Product Name
    Human Osteoprotegerin/TNFRSF11B (His Tag) recombinant protein
  • Documents
  • Description
    Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
  • Protein name
    Tumor necrosis factor receptor superfamily member 11B
  • Protein short names
    OSTEOPROTEGERIN; TR1; TNFRSF11B; OCIF; OPG; MGC29565
  • Uniprot ID
    O00300
  • Gene Name
    TNFRSF11B; OCIF; OPG
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the human TNFRSF11B (NP_002537.3) (Met 1-Leu 401) was fused with a polyhistidine tag at the C-terminus.
  • Protein Species
    Human
  • Molecular weight
    The secreted recombinant human TNFRSF11B comprises 391 amino acids and has a predicted molecular mass of 45.3 kDa. As a result of glycosylation, rhTNFRSF11B migrates as an approximately 55 kDa bnd in SDS-PAGE under reducing conditions.
  • Purity
    > 97 % as determined by SDS-PAGE
  • Activity
    1. Measured by its ability to inhibit TRAIL mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is typically 5-20 ng/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10 (Catalog # 10409-HNAE).
    2. Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF11B-His at 10 μg/ml (100 μl/well) can bind human Fc-TNFSF11 (Cat:11682-H01H) with a linear ranger of 3.125-200 ng/mL.
  • Validations

    Human Osteoprotegerin / TNFRSF11B Protein (His Tag) SDS-PAGE

    Human Osteoprotegerin / TNFRSF11B Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"