Human TRAIL R4/CD264/TNFRSF10D (His Tag) recombinant protein

Human TRAIL R4/CD264/TNFRSF10D (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Receptor for the cytotoxic ligand TRAIL. Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis. Reports are contradictory with regards to its ability to induce the NF-kappa-B pathway. According to PubMed:9382840, it cannot but according to PubMed:9430226, it can induce the NF-kappa-B pathway.

Tumor necrosis factor receptor superfamily member 10D

TRAIL-R4; TRAIL R4; TRAILR4; DCR2; CD264; TRUNDD

Q9UBN6

TNFRSF10D; DCR2; TRAILR4; TRUNDD; UNQ251/PRO288

Human Cells

A DNA sequence encoding the human TNFRSF10D (NP_003831.2) extracellular domain (Met 1-His 211) was fused with a polyhistidine tag at the C-terminus.

Human

The recombinant human TNFRSF10D consists of 167 amino acids after removal of the signal peptide and has a predicted a molecular mass of 18.4 kDa. As a result of glycosylation, the apparent molecular mass of rhTNFRSF10D is approximately 30-40 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF10D at 10 μg/ml (100 μl/well) can bind biotinylated TNFSF10 with a linear range of 0.625-40 ng/ml.
2. Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED₅₀ for this effect is typically 0.05-0.5 μg/mL in the presence of 20 ng/ml Recombinant Human TRAIL/TNFSF10.