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Gene name
T cell immunoreceptor with Ig and ITIM domains
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Organism
Mouse
- Entrez Gene ID
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Gene Symbol
Tigit
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Gene Type
protein coding
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Also Known as
Vstm3; ENSMUSG00000071552
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Encoded Protein Alias
T-cell immunoreceptor with Ig and ITIM domains; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; tigit
Accession NO.: NM_001146325
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Product Name
Tigit(NM_001146325) Mouse cDNA/ORF Clone
- Accession NO.
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Transcript Name
Mus musculus T cell immunoreceptor with Ig and ITIM domains (Tigit), mRNA.
- Synonyms
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ORF length
726
- DataSheet:
- DataSheet:
- FAQs:
Accession NO.: XM_017316834
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Product Name
Tigit(XM_017316834) Mouse cDNA/ORF Clone
- Accession NO.
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Transcript Name
PREDICTED: Mus musculus T cell immunoreceptor with Ig and ITIM domains (Tigit), transcript variant X1, mRNA.
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Synonyms
-
ORF length
711
- DataSheet:
- FAQs:
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Vector Name
pEXP-Entry
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primers
T7: TAATACGACTCACTATAGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4919
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Neomycin
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Protein Tag
Myc,FLAG
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Description
ORFs cloned in this vector will be expressed in mammalian cellsas a tagged protein with the C-terminal Myc-FLAG tags.
Such clones are the best for detection and purification of the transgene using anti-Myc or anti-FLAG antibodies.
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Vector Name
pGEM-T
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primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
3000
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Vector Type
Cloning Vector
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Promoter
lac
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
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Protein Tag
None
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Description
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
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Vector Name
pcDNA3.1-3xFlag
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
5508
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Ampicillin
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Selection Marker
Neomycin
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Protein Tag
Flag
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Description
Mammalian vector for expressing C-terminal 3xFlag tagged proteins.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"