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  • Mouse TIGIT/VSTM3 (His Tag) recombinant protein

Mouse TIGIT/VSTM3 (His Tag) recombinant protein

Cat.#: 501686

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Product Information

  • Product Name
    Mouse TIGIT/VSTM3 (His Tag) recombinant protein
  • Documents
  • Description
    TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-10 and diminished the production of IL-12p40. In addition, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.
  • Protein name
    T-cell immunoreceptor with Ig and ITIM domains
  • Protein short names
    VSIG9; ENSMUSG00000071552; TIGIT; DKFZP667A205; WUCAM; FLJ39873; VSTM3
  • Uniprot ID
    A0A0B4J1G6
  • Gene Name
    Tigit
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the extracellular domain of mouse TIGIT (NP_001139797.1) (Met 1-Gly 141) was expressed, with a C-terminal polyhistidine tag.
  • Protein Species
    Mouse
  • Molecular weight
    The secreted recombinant mouse TIGIT comprises 127 amino acids and has a calculated molecular mass of 14.2 kDa. As a result of glycosylation, the apparent molecular mass of rmSIRPA is approximately 20-25 kDa in SDS-PAGE under reducing conditions.
  • Purity
    > 90 % as determined by SDS-PAGE
  • Activity
    Immobilized mouse TIGIT-His at 10 μg/ml (100 μl/well) can bind mouse PVR-Fch (Cat:504218), The EC50 of mouse PVR-Fch (Cat:504218) is 0.31-0.73 μg/ml.
  • Validations

    Mouse TIGIT / VSTM3 Protein (His Tag) SDS-PAGE

    Mouse TIGIT / VSTM3 Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"