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Gene name
SMAD family member 1
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Organism
Human
- Entrez Gene ID
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Gene Symbol
SMAD1
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Gene Type
protein coding
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Also Known as
BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
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Encoded Protein Alias
mothers against decapentaplegic homolog 1; MAD homolog 1; MAD, mothers against decapentaplegic homolog 1; Mad-related protein 1; SMAD, mothers against DPP homolog 1; TGF-beta signaling protein 1; mothers against DPP homolog 1; transforming growth factor-beta signaling protein 1; transforming growth factor-beta-signaling protein 1
Accession NO.: NM_015583
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Product Name
SMAD1(NM_015583) Human cDNA/ORF Clone
- Accession NO.
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Transcript Name
Homo sapiens SMAD family member 1 (SMAD1), transcript variant 2, mRNA.
- Synonyms
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ORF length
1398
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Annotation
This variant (2) uses an alternate 5' exon compared to variant 1. Both variants encode the same protein.
- DataSheet:
- FAQs:
- DataSheet:
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- DataSheet:
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- DataSheet:
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Vector Name
pEXP-C-FLAG
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6013
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Hygromycin
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Protein Tag
FLAG
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Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-Entry
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primers
T7: TAATACGACTCACTATAGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4919
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Neomycin
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Protein Tag
Myc,FLAG
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Description
ORFs cloned in this vector will be expressed in mammalian cellsas a tagged protein with the C-terminal Myc-FLAG tags.
Such clones are the best for detection and purification of the transgene using anti-Myc or anti-FLAG antibodies.
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Vector Name
pEXP-untagged
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6223
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Ampicillin
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Selection Marker
Hygromycin
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Protein Tag
None
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Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pOTENT-1
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primers
pOT-F: TAATAGTAATCAATTACGGG
pOT-R: CCTCTACAAATGTGGTATGGC -
Vector Length
7510
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Neomycin
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Protein Tag
FLAG, 6 His
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Description
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags.
Such clones are the best for detection and purification of the transgene using anti-FLAG or anti-His antibodies.
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Vector Name
pUC18c
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primers
M13 fwd: GTAAAACGACGGCCAGT
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
2693
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Vector Type
Cloning Vector
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Promoter
lac
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
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Protein Tag
None
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Description
pMD18-T Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for this Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
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