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  • Rhesus IL-18 (His Tag) recombinant protein

Rhesus IL-18 (His Tag) recombinant protein

Cat.#: 504593

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Product Information

  • Product Name
    Rhesus IL-18 (His Tag) recombinant protein
  • Documents
  • Description
    Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.
  • Protein short names
    IL-1G; MGC12320; IL18; IL-18; IGIF; IL1F4
  • Uniprot ID
    Q9BGI5
  • Source/Expression Host
    E. coli
  • Expression Plasmid/cDNA
    A DNA sequence encoding the rhesus IL-18 (NP_001028006.1) (Ala 2-Asp 193) was expressed with an N-terminal polyhistidine tag.
  • Protein Species
    Rhesus
  • Molecular weight
    The recombinant rhesus IL18 consists of 203 amino acids and has a calculated molecular mass of 23.7 kDa.
  • Purity
    > 92 % as determined by SDS-PAGE
  • Activity
    1. Measured by its ability to bind human IL18R1 in a functional ELISA.
    2. Measured by its ability to induce IFNγsecretion by KG-1 human acute myelogenous leukemia cells in the presence of TNFα. The ED50 for this effect is typically 3-15 μg/mL in the presence of 20 ng/mL recombinant human TNFα.
  • Validations

    Cynomolgus IL-18 / IL-1F4 Protein (His Tag) SDS-PAGE

    Cynomolgus IL-18 / IL-1F4 Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"