Human VEGFR1/FLT-1 (His Tag) recombinant protein

Human VEGFR1/FLT-1 (His Tag) recombinant protein

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• Material Safety Data Sheets (MSDS)

Vascular endothelial growth factor receptor 1, also known as VEGFR-1, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor and FLT1, is a single-pass type I membrane protein and secreted protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR-1 / FLT1 contains seven Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. VEGFR-1 / FLT1 is expressed mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. It is specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR-1 / FLT1 is not expressed in tumor cell lines. VEGFR-1 / FLT1 is an essential receptor tyrosine kinase that regulates mammalian vascular development and embryogenesis. EGF-induced angiogenesis requires inverse regulation of VEGFR-1 and VEGFR-2 in tumor-associated endothelial cells. VEGFR-1 / FLT1 is a receptor for VEGF, VEGFB and PGF. It has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.

Receptor protein-tyrosine kinase

FLT-1; FLT; FLT1; AI323757; SFLT1; VEGFR-1; VEGFR1

P17948

FLT1

Human Cells

A DNA sequence encoding the human FLT1 (NP_002010.2) (Met1-Ile328) was expressed with a polyhistidine tag at the C-terminus.

Human

The recombinant human FLT1 consists 313 amino acids and predicts a molecular mass of 35.6 kDa.

> 90 % as determined by SDS-PAGE.

Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells ( HUVEC ). ( Conn, G. et al. ,1990 , Proc. Natl. Acad. Sci. USA 87:1323. )
The ED₅₀ for this effect is typically 10-40 ng/mL in the presence of 10 ng/mL human VEGF165.