Rat Ephrin-A5/EFNA5 (His Tag) recombinant protein

Rat Ephrin-A5/EFNA5 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Ephrin-A5 also known as EFNA5, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A5/EFNA5 may function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Ephrin-A5/EFNA5 also serves as a cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.

Ephrin-A5

EPLG7; EFL-5; AF1; GLC1M; EPHRIN-A5; LERK-7; AV158822; LERK7; EFNA5; EFL5; AL-1; RAGS; EPL7

P97605

Efna5; Eplg7; Lerk7

Human Cells

A DNA sequence encoding the rat EFNA5 (P97605) (Met 1-Glu 202), without the pro peptide, was expressed, fused with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat EFNA5 comprises 193 amino acids and predicts a molecular mass of 22.5 kDa. The apparent molecular mass of the ratEFNA5 is approximately 27 kDa in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized rat EFNA5-His at 10 μg/ml (100 μl/well) can bind rat EPHA3-Fc (Cat:501126), The EC₅₀ of rat EPHA3-Fc (Cat:501126) is 12.1-28.3 ng/ml.