Rat 4-1BBL / CD137L (Fc Tag) recombinant protein

Rat 4-1BBL / CD137L (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In additon, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.

Protein Tnfsf9

4-1BB-L; CD137L; 4-1BBL; TNFSF9; LY63L; AI848817

Q80WE6

Tnfsf9; rCG_44980

Human Cells

A DNA sequence encoding the rat TNFSF9 (Q80WE6) (Pro106-Gly308) was expressed, fused with Fc region of human IgG1 at the N-terminus.

Rat

The recombinant rat TNFSF9 comprises 463 amino acids and predicts a molecular mass of 50.9 kDa. The apparent molecular mass of the recombinant protein is approximately 61 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 96 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized canine TNFRSF9-His (Cat:504828) at 10 μg/ml (100 μl/well) can bind rat Fc-TNFSF9, The EC₅₀ of rat Fc-TNFSF9 is 0.26-0.62 μg/ml.