NovoPro
Cart 2
  • Ni NTA Beads 6FF

Ni NTA Beads 6FF

Cat.#: 800013

Special Price 110.8 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

Manuals & Protocols

NovoPro Ni NTA Beads 6 Fast Flow is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ion via a proprietary, chemical-stable pentadentate ligand. It is designed mainly for capturing and purification of histidine-tagged proteins secreted into eukaryotic cell (i.e. mammalian, and insect) culture supernatants. The strong nickel ion binding provides excellent resistance to EDTA and reducing agents such as DTT and β-ME. Ni-Penta Agarose enables direct loading of a large amount of culture sample, unlike conventional IMAC medium that requires a preceding step to remove nickel-chelating agents. Highly cross-linked 6% agarose as the base matrix support offers excellent mechanical stability for scaling up purification of histidine-tagged proteins.

Advantages

  • Fast, reliable scale-up of histidine-tagged protein purifications
  • High protein binding capacity, and minimal leakage of Ni2+ ions
  • Compatible with a very wide range of reducing agents, detergents, and other additives
  • Available as prepacked columns for added speed, convenience and reproducibility

Characteristics of NovoPro Ni NTA Beads 6 Fast Flow

Ni NTA Beads Ni NTA Beads 6FF
Matrix Cross-linked 4% agarose Highly cross-linked 6% agarose
Binding capacity >40mg 6XHis-tagged protein >40mg 6XHis-tagged protein
Mean particle size (μm) 45-165 45-165
Maximum back pressure 0.1 MPa, 1 bar 0.3 MPa, 3 bar
Storage 4°C - 30°C, 20% ethanol 4°C - 30°C, 20% ethanol

Compatibility

NovoPro Ni NTA 6 Fast Flow is compatible with the following reducing agents, denaturing agents, detergents, additives, and buffer substances (at least at the given concentrations)

Solution Concentration
Reducing agents 5 mM DTE
1 mM DTT
20 mM β-mercaptoethanol
5 mM TCEP
10 mM reduced glutathione
Denaturing agents 8 M urea
6 M Gua-HCl
Detergents 2% Triton™ X-100 (nonionic)
2% Tween™ 20 (nonionic)
2% NP-40 (nonionic)
2% Cholate (anionic)
1% CHAPS (zwitterionic)
Additives 500 mM imidazole
20% ethanol
50% glycerol
100 mM Na2SO4
1.5 M NaCl
1 mM EDTA
60 mM citrate
Buffer substances 50 mM sodium phosphate, pH 7.4
100 mM Tris-HCl, pH 7.4
100 mM Tris-acetate, pH 7.4
100 mM HEPES, pH 7.4
100 mM MOPS, pH 7.4
100 mM sodium acetate, pH 4

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"