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  • Human LCN2/NGAL (His Tag) recombinant protein

Human LCN2/NGAL (His Tag) recombinant protein

Cat.#: 502261

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Product Information

  • Product Name
    Human LCN2/NGAL (His Tag) recombinant protein
  • Documents
  • Description
    Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • Protein name
    Neutrophil gelatinase-associated lipocalin
  • Protein short names
    24P3; SIP24; MSFI; LCN2; AW212229; NGAL; RP23-161B9.11
  • Uniprot ID
    P80188
  • Gene Name
    LCN2; HNL; NGAL
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the human Lipocalin 2 precursor (NP_005555.2) (Met 1-Gly 198) was expressed with a C-terminal polyhistidine tag.
  • Protein Species
    Human
  • Molecular weight
    The secreted recombinant human LCN2 consists of 195 amino acids. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhLCN2 is approximately 22 kDa as predicted.
  • Purity
    > 97 % as determined by SDS-PAGE
  • Activity
    Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant human Lipocalin-2. Recombinant human Lipocalin-2 can bind >1.5 μM of Fe(DHBA)3.
  • Validations

    Human LCN2 / NGAL Protein (His Tag) SDS-PAGE

    Human LCN2 / NGAL Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"