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  • Ngf Mouse cDNA/ORF Clone

Ngf Mouse cDNA/ORF Clone

Cat.#: 709044-1

  • Accession#:
  • Vector:
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Special Price 267.6 USD

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Product Information

  • Gene name
    nerve growth factor
  • Organism
    Mouse
  • Entrez Gene ID
    18049
  • Gene Symbol
    Ngf
  • Gene Type
    protein coding
  • Also Known as
    Ngfb
  • Encoded Protein Alias
    beta-nerve growth factor; beta-NGF; nerve growth factor, beta
  • Product Name
    Ngf(NM_001112698) Mouse cDNA/ORF Clone
  • Accession NO.
    NM_001112698
  • Transcript Name
    Mus musculus nerve growth factor (Ngf), transcript variant 2, mRNA.
  • Synonyms
  • ORF length
    726
  • Annotation
    This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region compared to variant 1. The resulting isoform (B) has a shorter N-terminus compared to isoform A.
  • siRNA

    GAACAUUAACAACAGUGUAtt

    GCGUACAGGCAGAACCGUAtt

    GGUUGGAGAUAAGACCACAtt

  • DataSheet:
    NM_001112698 in pEXP-Entry
  • DataSheet:
    NM_001112698 in pUC18c
  • FAQs:
    DNA products or services FAQs
  • Product Name
    Ngf(NM_013609) Mouse cDNA/ORF Clone
  • Accession NO.
    NM_013609
  • Transcript Name
    Mus musculus nerve growth factor (Ngf), transcript variant 1, mRNA.
  • Synonyms
    XM_006501108  
  • ORF length
    924
  • Annotation
    This variant (1) represents the longer transcript and encodes the longer isoform (A).
  • siRNA

    GAACAUUAACAACAGUGUAtt

    GCGUACAGGCAGAACCGUAtt

    GGUUGGAGAUAAGACCACAtt

  • DataSheet:
    NM_013609 in pcDNA3.1-3xFlag
  • FAQs:
    DNA products or services FAQs
  • Vector Name
    pEXP-Entry
  • primers
    T7: TAATACGACTCACTATAGG
    M13 rev: CAGGAAACAGCTATGAC
  • Vector Length
    4919
  • Vector Type
    Mammalian Expression Vector
  • Promoter
    CMV
  • Antibiotic Resistance
    Kanamycin
  • Selection Marker
    Neomycin
  • Protein Tag
    Myc,FLAG
  • Description
    ORFs cloned in this vector will be expressed in mammalian cellsas a tagged protein with the C-terminal Myc-FLAG tags.
    Such clones are the best for detection and purification of the transgene using anti-Myc or anti-FLAG antibodies.
  • Vector Name
    pUC18c
  • primers
    M13 fwd: GTAAAACGACGGCCAGT
    M13 rev: CAGGAAACAGCTATGAC
  • Vector Length
    2693
  • Vector Type
    Cloning Vector
  • Promoter
    lac
  • Antibiotic Resistance
    Ampicillin
  • Selection Marker
    -
  • Protein Tag
    None
  • Description
    pMD18-T Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for this Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
  • Vector Name
    pcDNA3.1-3xFlag
  • primers
    T7: TAATACGACTCACTATAGGG
    BGH: TAGAAGGCACAGTCGAGG
  • Vector Length
    5508
  • Vector Type
    Mammalian Expression Vector
  • Promoter
    CMV
  • Antibiotic Resistance
    Ampicillin
  • Selection Marker
    Neomycin
  • Protein Tag
    Flag
  • Description
    Mammalian vector for expressing C-terminal 3xFlag tagged proteins.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"