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Product Name
Mouse CD10/Neprilysin (His Tag) recombinant protein
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Description
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) (By similarity). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).
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Protein name
Neprilysin
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Protein short names
CD10; NEP; NEUTRAL ENDOPEPTIDASE; 6030454K05RIK; MGC126707; NEPRILYSIN; SFE; MGC126681; MME; DKFZP686O16152; C85356; CALLA
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Uniprot ID
Q61391
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Gene Name
Mme
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Tyr 52-Trp 750) of mouse Mme (NP_032630.2) was fused with a polyhistidine tag at the N-terminus.
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Protein Species
Mouse
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Molecular weight
The secreted recombinant mouse Mme consists of 715 amino acids and has a calculated molecular mass of 82.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 90-100 kDa protein in SDS-PAGE under reducing conditions.
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Purity
> 98 % as determined by SDS-PAGE
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Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK-(Dnp)-OH, R&D Systems, Catalog # ES005.
The specific activity is > 3000 pmoles/min/μg. -
Validations
Mouse CD10 / Neprilysin / MME Protein (His Tag) SDS-PAGE
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