Human MAG/GMA/Siglec-4 (His Tag) recombinant protein

Human MAG/GMA/Siglec-4 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

The myelin-associated glycoprotein (MAG) contains five immunoglobulin-like domains and belongs to the sialic-acid-binding subgroup of the Ig superfamily. MAG is a transmembrane glycoprotein of 100kDa localized in myelin sheaths of periaxonal Schwann cell and oligodendroglial membranes where it functions in glia-axon interactions. It appears to function both as a receptor for an axonal signal that promotes the differentiation, maintenance and survival of oligodendrocytes and as a ligand for an axonal receptor that is needed for the maintence of myelinated axons. MAG contains a carbohydrate epitope shared with other glycoconjugates that is a target antigen in autoimmune peripheral neuropathy associated with IgM gammopathy and has been implicated in a dying back oligodendrogliopathy in multiple sclerosis. MAG is considered as a transmembrane protein of both CNS and PNS myelin and it strongly inhibits neurite outgrowth in both developing cerebellar and adult dosal root ganglion neurons. In contrast, MAG promotes neurite outgrowth from newborn DRG neurons. Thus, MAG may be responsible for the lack of CNS nerve regeneration and may influce both temporally and spatially regeneration in the PNS.

Myelin-associated glycoprotein

MGC123387; S-MAG; SIGLEC-4A; SIGLEC4A; GMA

P20916

MAG; GMA

Human Cells

A DNA sequence encoding the human MAG extracellular domain (P20916) (Met 1-Pro 516) was fused with a polyhistidine tag at the C-terminus.

Human

The secreted recombinant human MAG comprises 508 amino acids and has a predicted molecular mass of 56 kDa. The apparent molecular mass of rhMAG is approximately 85 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

1. Measured by its ability to bind mouse RTN4R-Fc2h (Cat:500415) in functional Elisa.
2. Measured by its ability to bind human RTN4R-Fch (Cat:503259) in functional Elisa.