Mouse SELP / P Selectin (His Tag) recombinant protein

Cat.#: 503628

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Product Information

  • Product Name
    Mouse SELP / P Selectin (His Tag) recombinant protein
  • Documents
  • Description
    P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.
  • Protein name
    P-selectin
  • Protein short names
    PLATELET ACTIVATION DEPENDENT GRANULE-EXTERNAL MEMBRANE PROTEIN; PSEL; CD62; P-SELECTIN; CD62 ANTIGEN-LIKE FAMILY MEMBER P; GMP-140; RP1-86F14.2; GMP140; GRANULE MEMBRANE PROTEIN 140; CD62P; PADGEM; GRMP; LEUKOCYTE-ENDOTHELIAL CELL ADHESION MOLECULE 3; LECAM3
  • Uniprot ID
    Q01102
  • Gene Name
    Selp; Grmp
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the mouse SELP (Q01102) (Met1-Ala709) was expressed with a C-terminal polyhistidine tag.
  • Protein Species
    Mouse
  • Molecular weight
    The recombinant mouse SELP comprises 679 amino acids and has a predicted molecular mass of 74 kDa. The apparent molecular mass of the protein is approximately 116 kDa in SDS-PAGE under reducing conditions.
  • Purity
    > 95 % as determined by SDS-PAGE
  • Activity
    Measured by the ability of the immobilized protein to support the adhesion of U937 cells.
    When 5 x 10E4 cells/well are added to SELP coated plates (1 μg/mL and 100 μL/well), approximately >85% cells will adhere specifically after 60 minutes at 37℃.
  • Validations

    Mouse SELP / selectin P / P-selectin Protein (His Tag) SDS-PAGE

    Mouse SELP / selectin P / P-selectin Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"