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Product Name
Mouse IL12A & IL12B (IL12A & IL12B Heterodimer) recombinant protein
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Uniprot ID
P43431
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the p35 subunit of mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse IL12 heterodimer of IL12A/IL12B, comprises 528 (204 + 324) amino acids and has a calculated molecular mass of 60.3 (23.1 + 37.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 28 & 47 kDa respectively in SDS-PAGE under reducing conditions.
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Purity
> 90 % as determined by SDS-PAGE
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Activity
1. Measured by its ability to bind biotinylated recombinant mouse IL12RB2 in a functional ELISA.
2. Measured in a cell proliferation assay using Anti-CD3-stimulated PBMC. The ED50 for this effect is typically 2-8 ng/mL. -
Validations
Mouse IL-12 (IL12A & IL12B Heterodimer) Protein SDS-PAGE
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