Mouse ICOS Ligand/B7-H2 (His & Fc Tag) recombinant protein

Mouse ICOS Ligand/B7-H2 (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains, and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. Present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production and germinal center formation.

Icos ligand

GL50-B; ICOSLG; LICOS; BG071784; GI50; LY115L; ICOS LIGAND; MKIAA0653; B7RP1; GL50; B7H2; ICOSL; B7H; B7RP-1; AU044799; KIAA0653; B7-H2; ICOS-L; CD275

Q9JHJ8

Icosl; mCG_5977

Human Cells

A DNA sequence encoding the mouse B7-H2 (NP_056605.1) extracellular domain (Met 1-Lys 279) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Mouse

The secreted recombinant mouse B7-H2/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer comprises 481 amino acids with a predicted molecular mass of 54.3 kDa. As a result of glycosylation, it migrates as an approximately 75-85 kDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized human ICOS at 1 μg/ml (100 μl/well) can bind biotinylated mouse B7-H2 Fc chimera with a linear range of 0.125-1.0 μg/ml.