Mouse Follistatin (His Tag) recombinant protein

Mouse Follistatin (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. follistatin are expressed and subserve local regulatory roles in numerous extragonadal tissues, including brain, adrenal, bone marrow, and placenta but perhaps most notably in anterior pituitary-the classical target tissue for inhibin, the activin-follistatin system may play a key role in early embryogenesis. Follistatin binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone follistatin is a binding protein to activin. Since activin binds to follistatin, it is imperative to determine the nature of the activin/follistatin binding complex.

Follistatin

P47931

Fst

Human Cells

A DNA sequence encoding the mouse FST (P47931) (Met 1-Trp 344) was expressed, fused with a polyhistidine tag at the C-terminus.

Mouse

The recombinant mouse FST comprises 326 amino acids and has a predicted molecular mass of 36.1 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFST is approximately 70-80 kDa due to glycosylation.

> 98 % as determined by SDS-PAGE

Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation.
The ED₅₀ for this effect is typically 0.02-0.08 μg/mL in the presence of 10 ng/ml Recombinant Human Activin A.