Mouse CTLA-4/CD152 (His Tag) recombinant protein

Mouse CTLA-4/CD152 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).

Cytotoxic T-lymphocyte protein 4

CH29-120A16.2; ALPS5; ICOS; CD; CTLA4; LY-56; CD152; CTLA-4; GRD4; CELIAC3; IDDM12; GSE

Q6GTR6

Ctla4; Cd152

Human Cells

A DNA sequence encoding the extracellular domain of mouse CTLA4 (NP_033973.2) (Met 1-Phe 162) was expressed, with a polyhistidine tag at the C-terminus.

Mouse

The recombinant mouse CTLA4 consists of 138 amino acids and has a predicted molecular mass of 15.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CTLA4 is approximately 25-30 kDa due to glycosylation.

> 98 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized recombinant mouse CTLA4-His (Cat:503267) at 10 μg/ml (100 μl/well) can mouse CD80-Fc (cat:502695) with a linear range of 0.78-62.5 ng/ml.
3. Immobilized recombinant mouse CTLA4-His (Cat:503267) at 10 μg/ml (100 μl/well) can human B7-1-Fc (Cat:503507) with a linear range of 0.78-125 ng/ml.