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  • Mouse CDC2 Kinase / CDK1 (His & GST Tag) recombinant protein

Mouse CDC2 Kinase / CDK1 (His & GST Tag) recombinant protein

Cat.#: 501223

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Product Information

  • Product Name
    Mouse CDC2 Kinase / CDK1 (His & GST Tag) recombinant protein
  • Documents
  • Description
    CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.
  • Protein short names
    CDC2A; MGC111195; CDC2> CDC28A; CDC2; DKFZP686L20222; P34CDC2; P34< CDK1
  • Uniprot ID
    P11440
  • Source/Expression Host
    Baculovirus-Insect Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the mouse CDK1 (P11440) (Met1-Met297) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
  • Protein Species
    Mouse
  • Molecular weight
    The recombinant mouse CDK1/GST chimera consists of 534 amino acids and has a calculated molecular mass of 61.9 kDa. The recombinant protein migrates as an approximately 57 kDa band in SDS-PAGE under reducing conditions.
  • Purity
    > 85 % as determined by SDS-PAGE
  • Activity
    Kinase activity untested
  • Validations

    Mouse CDC2 Kinase / CDK1 Protein (His & GST Tag) SDS-PAGE

    Mouse CDC2 Kinase / CDK1 Protein (His & GST Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"