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  • MBD3 antibody
  • MBD3 antibody
  • MBD3 antibody
  • MBD3 antibody

MBD3 antibody

Cat.#: 112503

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Special Price 239.0 USD

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Product Information

  • Product Name
    MBD3 antibody
  • Documents
  • Description
    MBD3 Rabbit Polyclonal antibody. Positive IP detected in mouse testis tissue. Positive WB detected in Y79 cells, COLO 320 cells, HeLa cells, Jurkat cells, MCF7 cells, mouse testis tissue. Positive IHC detected in human endometrial cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 33kd
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    MBD3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of MBD3 recombinant protein (Accession Number: BC043619). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:112503(MBD3 antibody) at dilution of 1:600

    Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:112503(MBD3 antibody) at dilution of 1:600

    Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:112503(MBD3 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human endometrial cancer using Catalog No:112503(MBD3 antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells, using MBD3 antibody Catalog No:112503 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of HepG2 cells, using MBD3 antibody Catalog No:112503 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    IP Result of anti-MBD3 (IP:Catalog No:112503, 4ug; Detection:Catalog No:112503 1:800) with mouse testis tissue lysate 4000ug.

    IP Result of anti-MBD3 (IP:Catalog No:112503, 4ug; Detection:Catalog No:112503 1:800) with mouse testis tissue lysate 4000ug.

  • Background
    MBD3 does not bind DNA by itself. It recruits histone deacetylases and DNA methyltransferases. MBD3 acts as transcriptional repressor and plays a role in gene silencing.(PMID:9774669) .
  • References
    • Ma P, Pan H, Montgomery RL, Olson EN, Schultz RM. Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proceedings of the National Academy of Sciences of the United States of America. 109(8):E481-9. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"